Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients
Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients
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DOI：
10.1111/j.1399-302x.2007.00406.x
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发表时间：
2008-04-01
影响因子：
--
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
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作者：
Nakano, K.;Inaba, H.;Ooshima, T.
简介：牙龈卟啉单胞菌是牙周病的主要致病菌，因其与心血管疾病的关系而受到越来越多的关注。根据编码菌毛亚基的fimA基因的多样性，将其菌毛分为I-V型和Ib型6种基因型。本研究分析牙龈卟啉单胞菌感染的心血管标本中fimA基因型分布。方法：收集心血管手术患者的112例心脏瓣膜和80例动脉粥样硬化斑块标本，以及56例牙菌斑标本。从每一个中提取细菌DNA，并用牙龈卟啉单胞菌特异性引物组进行聚合酶链反应分析。结果：10.4%的心血管标本和50.0%的牙菌斑标本中检出牙龈卟啉单胞菌;在后者中，II型是最常见的检测（35.7%），其次是I型（28.6%）和IV型（21.4%），而IV型和II型检测到相当大的频率为45.0%和30.0%，分别在心血管标本。相比之下，I型的发生是有限的（5.0%），在心血管samples.Conclusion：这些结果表明，特定的fimA基因型克隆，据报道与牙周炎，也经常窝藏在心血管标本，表明可能参与的II型和IV型克隆在心血管疾病的发生和发展。
Introduction: Porphyromonas gingivalis, a major periodontal pathogen, is gaining increasing attention for its possible association with cardiovascular diseases. Its fimbriae are classified into six genotypes (types I-V and Ib) based on the diversity of the fimA genes encoding the fimbrial subunits. In this study, fimA genotypic distribution was analyzed in P. gingivalis-infected cardiovascular specimens.Methods: A total of 112 heart valves and 80 atheromatous plaque specimens were collected from patients undergoing cardiovascular surgery, as well as 56 dental plaque specimens. Bacterial DNA was extracted from each, and polymerase chain reaction analysis was carried out with a P. gingivalis-specific set of primers. P. gingivalis-positive specimens were further analyzed to discriminate the fimA genotype using polymerase chain reaction with fimA type-specific primer sets.Results: P. gingivalis was detected in 10.4% of the cardiovascular specimens and 50.0% of the dental plaque samples. In the latter, type II was most frequently detected (35.7%), followed by types I (28.6%) and IV (21.4%), while types IV and II were detected with considerable frequencies of 45.0% and 30.0%, respectively, in the cardiovascular specimens. In contrast, the occurrence of type I was limited (5.0%) in the cardiovascular specimens.Conclusion: These results suggest that specific fimA genotypic clones, which are reportedly associated with periodontitis, are also frequently harbored in cardiovascular specimens, indicating the possible involvement of type II and IV clones in the initiation and progression of cardiovascular diseases.