ISOLATION OF CHROMOSOMAL ORIGINS OF REPLICATION IN YEAST
ISOLATION OF CHROMOSOMAL ORIGINS OF REPLICATION IN YEAST
复制标题
DOI：
10.1038/284185a0
复制
发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
SHALL, S
中科院分区：
文献类型：
--
作者：
BEACH, D;PIPER, M;SHALL, S
复制的起源已经在病毒的DNA、线粒体、细菌的质粒和细菌的染色体中被确定。然而，真核生物染色体复制的起源仍然难以捉摸，因为染色体的大尺寸和序列复杂性，特别是缺乏合适的检测方法。通过自主复制细胞质粒对酵母进行遗传转化的最新技术发展，现在使得以类似于用于细菌的方式寻找真核生物起源成为可能。在这里，我们描述了一个质粒的结构和性质，它不包含有效的真核生物复制起源，通过插入酵母染色体DNA的某些片段，其在酵母中的复制效率大大提高。我们相信这些含有复制起源，因为酵母转化体被证明含有转化质粒的拷贝。
Origins of replication have been identified in the DNA of viruses1, mitochondria2, bacterial plasmids3and the bacterial chromosome4. However, origins of replication of eukaryote chromosomes have remained elusive because of the large size and sequence complexity of chromosomes and in particular for want of a suitable assay for their detection. Recent development of techniques for genetic transformation of yeast by autonomously replicating cytoplasmic plasmids5–8now makes it possible to search for eukaryote origins in a manner analogous to that used for bacteria4. Here we describe the construction and properties of a plasmid which contains no effective eukaryote replication origin and whose efficiency of replication in yeast is greatly enhanced by insertion of certain fragments of yeast chromosomal DNA. We believe these to contain replication origins, since yeast transformants are shown to contain copies of the transforming plasmids.