MOLECULAR CLONING, EXPRESSION PATTERN OF MULTIDRUG RESISTANCE ASSOCIATED PROTEIN 1 (MRP1, ABCC1) GENE, AND THE SYNERGISTIC EFFECTS OF VERAPAMIL ON TOXICITY OF TWO INSECTICIDES IN THE BIRD CHERRY-OAT APHID
MOLECULAR CLONING, EXPRESSION PATTERN OF MULTIDRUG RESISTANCE ASSOCIATED PROTEIN 1 (MRP1, ABCC1) GENE, AND THE SYNERGISTIC EFFECTS OF VERAPAMIL ON TOXICITY OF TWO INSECTICIDES IN THE BIRD CHERRY-OAT APHID
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樱桃燕麦蚜多药耐药相关蛋白1（mrp1、abcc1）基因的分子克隆、表达模式及维拉帕米对两种杀虫剂毒性的协同作用
DOI：
10.1002/arch.21334
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发表时间：
2016-05-01
影响因子：
2.2
通讯作者：
Chen, Mao-Hua
中科院分区：
文献类型：
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作者：
Kang, Xin-Le;Zhang, Meng;Chen, Mao-Hua
ATP结合盒（ABC）转运蛋白是一类重要的跨膜蛋白，由一个超基因家族编码。大多数ABC蛋白是主要的主动转运蛋白，其结合并水解ATP以介导多种底物穿过脂质膜的流出。在这项研究中，我们克隆和鉴定了一个假定的多药耐药相关蛋白1（MRP 1）从禾谷缢管蚜ABCC 1编码。结构分析表明，该蛋白具有ABC转运蛋白家族的典型结构特征。系统进化分析表明，该蛋白与豌豆蚜的相应蛋白氨基酸序列高度相似。实时荧光定量PCR分析表明，ABCC 1在所有的R. Padi发育阶段，在第四龄幼虫中表达水平最高。我们还检查了ABCC1在四种不同组织类型中的表达，发现它在中肠中表达最高。暴露河水稻对吡虫啉和毒死蜱的敏感性增加了ABCC1的表达。此外，ABCC1在吡虫啉抗性（IR）和毒死蜱抗性（CR）菌株中的表达高于在对杀虫剂敏感的菌株中的表达。帕迪。暴露河Padi对维拉帕米与杀虫剂的组合显著增加了杀虫剂的毒性。CR和IR R各自的协同因子。padi品系的平均值分别为1.33和1.26，低于SS品系的平均值（2.72和1.64）。我们的研究结果阐明了ABCC 1在R. padi，特别是其在杀虫剂抗性中的作用，并建议使用ABC转运蛋白抑制剂，以提高杀虫剂的有效性害虫管理的新策略。(C)2016 Wiley Periodicals，Inc.
The ATP-binding cassette (ABC) transporters are important transmembrane proteins encoded by a supergene family. The majority of ABC proteins are primary active transporters that bind and hydrolyze ATP to mediate the efflux of a diverse range of substrates across lipid membranes. In this study, we cloned and characterized a putative multidrug resistance associated protein 1 (MRP1) from Rhopalosiphum padi encoded by ABCC1. Structural analysis showed that this protein has structural features typical of the ABC transporter family. Phylogenetic analysis indicated that the amino acid sequence was highly similar that of the corresponding protein from Acyrthosiphon pisum. Real-time quantitative polymerase chain reaction (PCR) analysis showed that ABCC1 was expressed throughout all R. padi developmental stages, with the highest level of expression in the fourth larval instar. We also examined ABCC1 expression in four different tissue types and found that it was most highly expressed in the midgut. Exposing R. padi to imidacloprid and chlorpyrifos increased ABCC1 expression. Furthermore, ABCC1 expression was higher in the imidacloprid-resistant (IR) and chlorpyrifos-resistant (CR) strains than in an insecticide-susceptible strain (SS) of R. padi. Exposing R. padi to verapamil in combination with insecticides significantly increased the toxicity of the insecticides. The respective synergy factor of CR and IR R. padi strain was 1.33 and 1.26, which was lower than that (2.72 and 1.64, respectively) of the SS. Our results clarify the biological function of ABCC1 in R. padi, particularly its role in insecticide resistance, and suggest novel strategies for pest management that use ABC transporter inhibitors to increase the effectiveness of insecticides. (C) 2016 Wiley Periodicals, Inc.