Viroids:: the minimal non-coding RNAs with autonomous replication
Viroids:: the minimal non-coding RNAs with autonomous replication
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DOI：
10.1016/j.febslet.2004.03.118
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发表时间：
2004-06-01
期刊：
影响因子：
3.5
通讯作者：
De la Peña, M
中科院分区：
文献类型：
--
作者：
Flores, R;Delgado, S;De la Peña, M
类病毒是小的（246-401个核苷酸），非编码的环状rna，能够在某些植物中自主复制。类病毒分为类病毒科和类病毒科，其成员分别在细胞核和叶绿体中进行复制。复制通过基于RNA的滚环机制发生，分三个步骤：(1)由宿主dna依赖的RNA聚合酶催化合成超过单位长度的链，迫使转录RNA模板；(2)加工到单位长度，在avsunviidae家族中由锤头核酶介导；(3)通过RNA连接酶或自催化进行环化。疾病诱导可能是由于类病毒特异性小干扰RNA的积累，通过RNA沉默，可以干扰正常的发育途径。
Viroids are small (246–401 nucleotides), non-coding, circular RNAs able to replicate autonomously in certain plants. Viroids are classified into the families Pospiviroidae and Avsunviroidae, whose members replicate in the nucleus and chloroplast, respectively. Replication occurs by an RNA-based rolling-circle mechanism in three steps: (1) synthesis of longer-than-unit strands catalyzed by host DNA-dependent RNA polymerases forced to transcribe RNA templates, (2) processing to unit-length, which in family Avsunviroidae is mediated by hammerhead ribozymes, and (3) circularization either through an RNA ligase or autocatalytically. Disease induction might result from the accumulation of viroid-specific small interfering RNAs that, via RNA silencing, could interfere with normal developmental pathways.