Hydrogen Sulfide Production From Cysteine and Homocysteine by Periodontal and Oral Bacteria
Hydrogen Sulfide Production From Cysteine and Homocysteine by Periodontal and Oral Bacteria
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DOI：
10.1902/jop.2009.090012
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发表时间：
2009-11-01
影响因子：
4.3
通讯作者：
Nakayama, Koji
中科院分区：
文献类型：
--
作者：
Yoshida, Akihiro;Yoshimura, Mamiko;Nakayama, Koji
背景：硫化氢是口腔细菌产生的主要挥发性硫化物之一。本研究建立了口腔细菌产生硫化氢的检测系统，并对该系统进行了评价。方法：利用L-methionine-alpha-deamino-gamma-mercaptomethane-lyase酶(METase)和βC-硫裂解酶(βC-S)分别降解同型半胱氨酸和半胱氨酸产生硫化氢。利用三氯化铋与硫化氢混合生成黑色沉淀物的反应，对产生硫化氢的酶反应进行了分析。结果：以L-半胱氨酸和同型半胱氨酸为底物，产硫化氢的口腔链球菌FW73产硫化氢最多，而牙龈卟啉单胞菌美国典型培养物33277和W83和核梭杆菌ATCC 10953产硫化氢最多。最后对龈下菌斑中发现的硫化氢进行了分析。结论：用三氯化铋可以方便地分析口腔细菌产生的硫化氢。然而，要实际使用，还需要进一步的创新。月经周期2009，80：1845-1851。
Background: Hydrogen sulfide is one of the predominant volatile sulfur compounds (VSCs) produced by oral bacteria. This study developed and evaluated a system for detecting hydrogen sulfide production by oral bacteria.Methods: L-methionine-alpha-deamino-gamma-mercaptomethane-lyase (METase) and beta carbon-sulfur (beta C-S) lyase were used to degrade homocysteine and cysteine, respectively, to produce hydrogen sulfide. Enzymatic reactions resulting in hydrogen sulfide production were assayed by reaction with bismuth trichloride, which forms a black precipitate when mixed with hydrogen sulfide. The enzymatic activities of various oral bacteria that result in hydrogen sulfide production and the capacity of bacteria from periodontal sites to form hydrogen sulfide in reaction mixtures containing L-cysteine or DL-homocysteine were assayed.Results: With L-cysteine as the substrate, Streptococcus anginosus FW73 produced the most hydrogen sulfide, whereas Porphyromonas gingivalis American Type Culture Collection (ATCC) 33277 and W83 and Fusobacterium nucleatum ATCC 10953 produced similar to 35% of the amount produced by the P. gingivalis strains. Finally, the hydrogen sulfide found in subgingival plaque was analyzed. Using bismuth trichloride, the hydrogen sulfide produced by oral bacteria was visually detectable as a black precipitate.Conclusions: Hydrogen sulfide production by oral bacteria was easily analyzed using bismuth trichloride. However, further innovation is required for practical use. J Periodontol 2009, 80:1845-1851.