Electrochemiluminescence immunosensor for the determination of ag alpha fetoprotein based on energy scavenging of quantum dots
Electrochemiluminescence immunosensor for the determination of ag alpha fetoprotein based on energy scavenging of quantum dots
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DOI：
10.1016/j.elecom.2011.10.011
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发表时间：
2012
影响因子：
5.4
通讯作者：
Zhiyong Guo;Tingting Hao;Sui Wang;Ning Gan;Xing Li;Danyi Wei
中科院分区：
文献类型：
--
作者：
Zhiyong Guo;Tingting Hao;Sui Wang;Ning Gan;Xing Li;Danyi Wei
以石墨烯-硫化镉量子点-海藻酸盐(G-CDS量子点-AL)为固定载体，以硫化镉/硫化锌量子点为标记物，制备了用于甲胎蛋白(AFP)超灵敏检测的电化学发光免疫传感器。CdSe/ZnS量子点能有效清除G-CDS量子点-Al复合材料的电致发光，其猝灭电致发光强度与AFP浓度在0.05~500fg/mL范围内的对数呈线性关系。方法的检出限为20g/m L。所建立的检测AFP的ECL免疫分析方法稳定、特异、灵敏度高，具有良好的临床应用前景。
An electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of alpha fetoprotein (AFP) was fabricated using graphene-CdS quantum dots-alginate (G-CdS QDs-AL) as the immobilizing support and CdSe/ZnS QDs as the label. CdSe/ZnS QDs could effectively scavenge the ECL of G-CdS QDs-AL composite, and the quenched ECL intensity depended linearly on the logarithm for AFP concentration in the range from 0.05 to 500fg/mL. The detection limit was 20ag/mL. The proposed ECL immunoassay protocol for AFP detection is stable, specific, highly sensitive and promising for clinical application.