Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein - Nitrilotriacetic acid conjugate
Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein - Nitrilotriacetic acid conjugate
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DOI：
10.1021/ja0559754
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发表时间：
2006-01-18
影响因子：
15
通讯作者：
Lippard, SJ
中科院分区：
文献类型：
--
作者：
Goldsmith, CR;Jaworski, J;Lippard, SJ
化合物NTA - DCF由两部分组成，二氯荧光素（DCF）报告因子和硝三乙酸（NTA）功能。后者通过桥接金属离子选择性地结合多组氨酸序列。NTA - DCF共轭物本身及其镍（II）配合物具有与母体DCF荧光团相似的光物理性质。对顺磁离子的发射不敏感使探针能够在HEK 293-T和HeLa细胞的细胞外表面荧光标记his6标记的蛋白质。
The compound NTA−DCF consists of two components, a dichlorofluorescein (DCF) reporter and a nitrilotriacetic acid (NTA) functionality. The latter binds polyhistidine sequences selectively through a bridging metal ion. The NTA−DCF conjugate has photophysical properties similar to those of the parent DCF fluorophore both by itself and as its nickel(II) complex. The insensitivity of the emission to paramagnetic ions allows the probe to label His6-tagged proteins fluorescently on the extracellular surfaces of HEK 293-T and HeLa cells.