ASB-1, a germline-specific isoform of mitochondrial ATP synthase b subunit, is required to maintain the rate of germline development in Caenorhabditis elegans
ASB-1, a germline-specific isoform of mitochondrial ATP synthase b subunit, is required to maintain the rate of germline development in Caenorhabditis elegans
复制标题
DOI：
10.1016/j.mod.2006.11.004
复制
发表时间：
2007-03-01
影响因子：
2.6
通讯作者：
Iino, Yuichi
中科院分区：
文献类型：
--
作者：
Kawasaki, Ichiro;Hanazawa, Momoyo;Iino, Yuichi
所有类型细胞的发育时间必须同步和空间协调，以实现多细胞生物体的有组织发育。在此之前，我们发现编码线粒体ATP合成酶B亚基的种系特异性异构体asB-1的RNAi在秀丽隐杆线虫中引起100%的渗透不育。ATP合酶是线粒体呼吸链的五种复合物之一，已知该链的某些组分的缺陷会减缓蠕虫的生长并延长其寿命。我们发现，asb-1突变体生殖系的发育在任何阶段都没有被阻止，但确实减慢到野生型的一半，而asb-1突变体的体细胞发育速率与野生型相同，这表明asb-1是维持生殖系发育速率所必需的，但对体细胞发育没有影响。在ATP合酶亚基基因中，编码g亚基的种系特异性同种型的asg-1的RNAi也引起asb-1样不育，表明还需要一些其他种系特异性组分来维持种系发育的速率。asb-1和asg-1都位于常染色体上，而它们分别在X染色体上拥有对应物asb-2和asg-2，这两者都是体细胞发育所需的。基因的染色体位置可能是每个基因的生殖系/体细胞功能分离的基础，如其他常染色体/X连锁重复基因对所示。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
The developmental timing of all types of cells must be synchronized and spatially coordinated to achieve the organized development of a multicellular organism. Previously, we found RNAi of asb-1, encoding a germline-specific isoform of mitochondrial ATP synthase b subunit, caused 100% penetrant sterility in Caenorhabditis elegans. ATP synthase is one of the five complexes of the mitochondrial respiratory chain, and defects in some of the components of the chain are known to slow the growth and extend the lifespan of worms. We found that development of asb-1 mutant germ line was not arrested at any stage, but did slow to half the rate of wild type, whereas the rate of somatic development was the same in asb-1 mutants as that of wild type, indicating that asb-1 is required to maintain the rate of germline development but has no effect on somatic development. Among ATP synthase subunit genes, RNAi of asg-1, encoding a germline-specific isoform of the g subunit, also caused asb-l-like sterility, indicating that some other germline-specific components are also required to maintain the rate of germline development. Both asb-1 and asg-1 are located on autosomes while they possess counterparts, asb-2 and asg-2, respectively, on X chromosome, which are both required for somatic development. Chromosomal locations of the genes may be the basis of the segregation of germline/somatic functions of each gene, as were demonstrated for other autosomal/X-linked duplicated gene pairs. (c) 2006 Elsevier Ireland Ltd. All rights reserved.