STUDIES ON THE SEQUENCE AND STRUCTURE OF THE ESCHERICHIA-COLI K-12 NUPG GENE, ENCODING A NUCLEOSIDE-TRANSPORT SYSTEM
STUDIES ON THE SEQUENCE AND STRUCTURE OF THE ESCHERICHIA-COLI K-12 NUPG GENE, ENCODING A NUCLEOSIDE-TRANSPORT SYSTEM
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DOI：
10.1111/j.1432-1033.1987.tb13431.x
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发表时间：
1987-10-15
期刊：
影响因子：
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通讯作者：
MUNCHPETERSEN, A
中科院分区：
文献类型：
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作者：
HANSEN, SEW;JENSEN, N;MUNCHPETERSEN, A
已将编码大肠杆菌K-12中两种活性核苷转运系统之一的nupG基因克隆到多拷贝质粒pBR 322及其衍生物上。重组质粒补充了染色体nupG突变。通过限制性内切酶消化确定了遗传图谱，并在克隆到M13 cDNA中的片段上确定了3-kb DNA片段的核苷酸序列。一个1254 bp的开放阅读框，编码一个计算分子量为45.333 kDa的蛋白质，被推断为nupG的编码区。携带含有该基因的质粒的微细胞形成菌株被证明产生一种表观分子量约为43 kDa的疏水性膜结合多肽。
ThenupGgene, encoding one of the two active nucleoside‐transport systems inEscherichia coliK‐12, has been cloned on the multicopy plasmid pBR322 and derivatives thereof. The recombinant plasmids complemented a chromosomalnupGmutation.A genetic map was determined by digestion with restriction endonucleases and the nucleotide sequence of a 3‐kb stretch of DNA has been determined on fragments cloned into M13 phages. An open reading frame of 1254 bp, encoding a protein with a calculated molecular mass of 45.333 kDa, was deduced to be the coding region ofnupG.Minicell‐forming strains carrying plasmids containing this gene were shown to produce a hydrophobic, membrane‐bound polypeptide with an apparent molecular mass of approximately 43 kDa.