On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment
On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment
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DOI：
10.3390/mi6050648
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发表时间：
2015-05-01
期刊：
影响因子：
3.4
通讯作者：
Arai, Fumihito
中科院分区：
文献类型：
--
作者：
Nakahara, Kou;Sakuma, Shinya;Arai, Fumihito
我们提出了一个系统，运输卵母细胞和测量其机械特性在一个开放的环境中使用机器人集成微流控芯片（芯片）。细胞通过芯片中的微柱阵列传输，并通过机械探针和力传感器测量其特性。由于芯片具有开放的微通道，因此很容易引入和收集卵母细胞等重要细胞，而不会有丢失它们的风险。此外，芯片中捕获的任何气泡（降低测量精度）都很容易去除。为了通过开放的微通道运输卵母细胞，我们采用了基于振动诱导流动的运输技术。在这种流动下，卵母细胞到达测量点，在那里它们的机械特性被确定。我们演示了引进，运输，机械特性的测量，并使用该系统收集卵母细胞。
We propose a system that transports oocytes and measures their mechanical characteristics in an open environment using a robot integrated microfluidic chip (chip). The cells are transported through a micropillar array in the chip, and their characteristics are measured by a mechanical probe and a force sensor. Because the chip has an open microchannel, important cells such as oocytes are easily introduced and collected without the risk for losing them. In addition, any bubbles trapped in the chip, which degrade the measurement precision, are easily removed. To transport the oocytes through the open microchannel, we adopt a transportation technique based on a vibration-induced flow. Under this flow, oocytes arrive at the measurement point, where their mechanical characteristics are determined. We demonstrate the introduction, transportation, measurement of mechanical characteristics, and collection of oocytes using this system.