Insight into nitrogen removal performance of anaerobic ammonia oxidation in two reactors: Comparison based on the aspects of extracellular polymeric substances and microbial community
Insight into nitrogen removal performance of anaerobic ammonia oxidation in two reactors: Comparison based on the aspects of extracellular polymeric substances and microbial community
复制标题
两个反应器厌氧氨氧化脱氮性能的洞察：基于胞外聚合物和微生物群落方面的比较
DOI：
10.1016/j.bej.2022.108526
复制
发表时间：
2022-06
影响因子：
3.9
通讯作者：
Xuliang Zhuang
中科院分区：
文献类型：
--
作者：
Dongmin Yang;Cancan Jiang;Shengjun Xu;Likun Gu;Danhua Wang;Jialiang Zuo;Huacai Wang;Shujun Zhang;Dongsheng Wang;Huanzhen Zhang;Xuliang Zhuang
厌氧氨氧化（Anammox）技术有望在处理低碳氮比废水中发挥重要作用。本研究通过16S rRNA高通量测序，研究了两种不同反应器的脱氮性能和胞外聚合物质（EPS）对厌氧氨氧化活性的影响。序批式反应器（SBR）和上流式厌氧氨氧化污泥膜式反应器（UASB）运行133 d，三期总氮去除率分别为82.09±4.76%和87.91±2.59%。UASB中EPS浓度约为SBR的1.6倍。同时，在批量试验中，特别是UASB的厌氧氨氧化-EPS （A U -EPS）的添加对厌氧氨氧化菌（AnAOB）的脱氮能力有较好的促进作用。最终观察到UASB中AnAOB的相对丰度达到19.68%，在整个群落中占比较高的程度。在SBR中，随着底物浓度的增加，出水nh4 + -N浓度升高，AnAOB的相对丰度随之降低。因此，不同的投料策略会导致不同的造粒方式，从而影响厌氧氨氧化系统的活性和稳定性。本研究旨在更好地了解与AnAOB富集策略相关的不同机制。•anammox - eps的加入有效提高了厌氧氨氧化活性。•添加UASB的Anammox-EPS可提高SAA。•与SBR相比，UASB的生物量保持能力更高。
Anaerobic ammonium oxidation (Anammox) is expected to play a promising role in treating low C/N ratio wastewater. In this study, nitrogen removal performance and the effects of extracellular polymeric substances (EPS) on anammox activity were investigated in two different reactors, followed by analysis of 16S rRNA high-throughput sequencing. A sequencing batch reactor (SBR) and an up-flow anammox sludge blanket reactor (UASB) operated for 133 days, with total nitrogen removal efficiencies of 82.09 ± 4.76% and 87.91 ± 2.59% in the phase 3, respectively. The EPS concentration in UASB was about 1.6 times higher than that in SBR. Also, in the batch tests, especially the anammox-EPS of UASB (A U -EPS) additions contributed to better nitrogen removal capability for anammox bacteria (AnAOB). The relative abundance of AnAOB in UASB was observed to reach 19.68% in the end, accounting for a relatively high degree in the whole community. In SBR, the effluent NH 4 + -N concentration increased when increasing the substrate concentration, followed by the decrease of the relative abundance of AnAOB. Thus, the different feeding strategies would lead to different granulation styles, further influencing the activity and stability of anammox system. This research aims to get a better understanding of the different mechanisms associated with AnAOB enrichment strategies. • Addition of Anammox-EPS effectively enhanced the anammox activity. • Adding the Anammox-EPS of UASB led to a higher SAA. • Compared to SBR, there was a higher biomass retention ability in UASB.