SOLID2: An Antibody Array-Based Life-Detector Instrument in a Mars Drilling Simulation Experiment (MARTE)
SOLID2: An Antibody Array-Based Life-Detector Instrument in a Mars Drilling Simulation Experiment (MARTE)
复制标题
DOI：
10.1089/ast.2007.0126
复制
发表时间：
2008-10-01
期刊：
影响因子：
4.2
通讯作者：
Stoker, Carol R.
中科院分区：
文献类型：
--
作者：
Parro, Victor;Fernandez-Calvo, Patricia;Stoker, Carol R.
基于抗体阵列的生命探测器仪器的现场原型，生命迹象探测器（SOLID 2），已经在一个名为MARTE（火星天体生物学研究和技术实验）的火星钻探使命模拟中进行了测试。作为MARTE机器人钻机上的分析仪器之一，SOLID 2使用含有157种不同抗体的蛋白质微阵列对研磨岩心样品（0.5 g）进行自动样品处理和分析。从不同深度（下至5.5米）的岩心样品进行了分析，并获得阳性反应的抗体提出了对革兰氏阴性细菌氧化亚铁纤溶酶，一种属Acidithiobacillus（两种常见的微生物在力拓地区），并提取物从生物膜和其他天然样品从力拓地区。这些阳性反应在样品之前进行高温处理时不存在，这表明抗体-抗原反应的生物来源和结构依赖性。我们的结论是，像SOLID 2这样的基于抗体阵列的生命探测器仪器可以检测复杂的生物材料，它应该被认为是未来寻找生命的行星任务的潜在分析仪器。
A field prototype of an antibody array-based life-detector instrument, Signs of Life Detector (SOLID2), has been tested in a Mars drilling mission simulation called MARTE (Mars Astrobiology Research and Technology Experiment). As one of the analytical instruments on the MARTE robotic drilling rig, SOLID2 performed automatic sample processing and analysis of ground core samples (0.5 g) with protein microarrays that contained 157 different antibodies. Core samples from different depths (down to 5.5 m) were analyzed, and positive reactions were obtained in antibodies raised against the Gram-negative bacterium Leptospirillum ferrooxidans, a species of the genus Acidithiobacillus (both common microorganisms in the Rio Tinto area), and extracts from biofilms and other natural samples from the Rio Tinto area. These positive reactions were absent when the samples were previously subjected to a high-temperature treatment, which indicates the biological origin and structural dependency of the antibody-antigen reactions. We conclude that an antibody array-based life-detector instrument like SOLID2 can detect complex biological material, and it should be considered as a potential analytical instrument for future planetary missions that search for life.