The application of functional silica nanoparticles to fulfill the rapid and improved enantioselective capillary electrophoresis separation of amino acid derivatives.
The application of functional silica nanoparticles to fulfill the rapid and improved enantioselective capillary electrophoresis separation of amino acid derivatives.
复制标题
DOI：
10.1002/jssc.201401016
复制
发表时间：
2015
影响因子：
3.1
通讯作者：
Bingxiang Wang;Weibo Chai;Guosheng Ding
中科院分区：
文献类型：
--
作者：
Bingxiang Wang;Weibo Chai;Guosheng Ding
本研究成功合成了粒径为118 ± 12 nm的二氨基功能化二氧化硅纳米粒子，并将其直接引入到手性毛细管电泳体系中，以去甲万古霉素为手性选择剂，对9-芴甲氧羰基衍生的氨基酸进行手性拆分。在酸性背景电解质条件下，功能性二氧化硅纳米粒子可以通过静电相互作用吸附到裸二氧化硅毛细管柱的内表面，形成动态涂层，从而产生反向阳极电渗流（即从阴极到阳极）。如预期的那样，手性氨基酸衍生物（通常带负电荷）可以在共电渗流条件下在当前分离系统中快速分离。此外，由于去甲万古霉素手性选择剂在毛细管壁上的吸附被大大抑制，对映体分离的柱性能和检测灵敏度也得到明显提高。考察了涂层厚度、背景电解质浓度、功能性二氧化硅纳米粒子浓度、有机改性剂等因素对分离的影响，得到了最佳分离条件。
In this study, diamino moiety functionalized silica nanoparticles with the size of 118 ± 12 nm were successfully synthesized and directly introduced into a chiral capillary electrophoresis system to improve the enantioseparation of 9-fluorenyl methoxycarbonyl derivatized amino acids using norvancomycin as chiral selector. Under acidic background electrolyte conditions, functional silica nanoparticles can be readily adsorbed onto the inner surface of bare silica capillary column through electrostatic interaction to form a dynamic coating, resulting in a reversed anodic electro-osmotic flow (i.e. from cathode to anode). As expected, chiral amino acid derivatives (usually negatively charged) can be rapidly separated under co-electro-osmotic flow conditions in the current separation system. Furthermore, the column performance and detection sensitivity for the enantioseparation were also obviously improved because the adsorption of chiral selector of norvancomycin to the capillary wall was greatly suppressed. Some important factors influencing the separation, such as the coating thickness, background electrolyte concentration, functional silica nanoparticles concentration, and the organic modifier were also investigated and the optimized separation conditions were obtained.