Effects of osteogenesis on dermal fibroblasts cultured in vitro.
Effects of osteogenesis on dermal fibroblasts cultured in vitro.
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DOI：
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发表时间：
1993-11
影响因子：
6.1
通讯作者：
R. Xu;H. Rao;Y. Zhu;B. Chai
中科院分区：
文献类型：
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作者：
R. Xu;H. Rao;Y. Zhu;B. Chai
从离体培养的新西兰兔裂厚皮肤中获得成纤维细胞。成纤维细胞传代8天后，培养瓶内可见不透明的骨结节。在成纤维细胞传代培养37天后，一些结节可能扩大延伸并形成小梁样或片状结构。在紫外光荧光显微镜下，经四环素静脉标记后，上述结构呈金黄色荧光，说明为新形成的骨组织。这些结构的钙盐和胶原染色均呈阳性反应，充分反映了钙和胶原的组成。体外培养的真皮成纤维细胞可诱导成骨。
Fibroblast were harvested from in vitro culture of split-thickness skin of New Zealand rabbit. Some opaque bone nodules were found in the culture flasks when the fibroblasts underwent subculture for 8 days. After a 37-day subculture of fibroblasts, some of the nodules might enlarge extend and form trabecular-like or flake-like structures. The above-mentioned structures revealed golden-yellow fluorescence under the blueviolet light fluorescence microscope when the specimens were labeled with tetracycline intravitally, denoting that they were newly formed bone tissues. Both calcium salt and collagen stainings for these structures showed positive reaction, fully reflecting the calcium and collagen composition. Osteogenesis caused by dermal fibroblast cultured in vitro was thus verified.