Industrial Relevance of Chromosomal Copy Number Variation in Saccharomyces Yeasts.
Industrial Relevance of Chromosomal Copy Number Variation in Saccharomyces Yeasts.
复制标题
DOI：
10.1128/aem.03206-16
复制
发表时间：
2017-06-01
影响因子：
4.4
通讯作者：
Daran JG
中科院分区：
文献类型：
--
作者：
Gorter de Vries AR;Pronk JT;Daran JG
染色体拷贝数变异（CCNV）在真核生物的进化和健康中起着关键作用。单细胞酵母（Saccharomyces cerevisiae）是研究CCNV发生、生理影响和进化意义的重要模型。对这种酵母的基础研究为分析和引入CCNV提供了广泛的方法。此外，这些研究提供了在进化背景下CCNV的消极和积极影响之间的平衡的见解。越来越多的证据表明，CCNV不仅经常发生在酵母菌的工业菌株中，而且是工业相关性状多样性的关键贡献者。在进化工程中获得的工业相关性状中，CCNV的频繁参与进一步支持了这一概念。本文介绍了基因组测序和基因组编辑技术的最新进展，并讨论了这些技术如何为揭示CCNV在工业酵母菌株中的作用以及合理设计酵母染色体拷贝数和核型提供机会。
Chromosomal copy number variation (CCNV) plays a key role in evolution and health of eukaryotes. The unicellular yeast Saccharomyces cerevisiae is an important model for studying the generation, physiological impact, and evolutionary significance of CCNV. Fundamental studies of this yeast have contributed to an extensive set of methods for analyzing and introducing CCNV. Moreover, these studies provided insight into the balance between negative and positive impacts of CCNV in evolutionary contexts. A growing body of evidence indicates that CCNV not only frequently occurs in industrial strains of Saccharomyces yeasts but also is a key contributor to the diversity of industrially relevant traits. This notion is further supported by the frequent involvement of CCNV in industrially relevant traits acquired during evolutionary engineering. This review describes recent developments in genome sequencing and genome editing techniques and discusses how these offer opportunities to unravel contributions of CCNV in industrial Saccharomyces strains as well as to rationally engineer yeast chromosomal copy numbers and karyotypes.