Real-time cell analysis of the cytotoxicity of a pH-responsive drug-delivery matrix based on mesoporous silica materials functionalized with ferrocenecarboxylic acid
Real-time cell analysis of the cytotoxicity of a pH-responsive drug-delivery matrix based on mesoporous silica materials functionalized with ferrocenecarboxylic acid
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基于二茂铁甲酸功能化介孔二氧化硅材料的 pH 响应药物递送基质的细胞毒性的实时细胞分析
DOI：
10.1016/j.aca.2018.11.017
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发表时间：
2019-03-21
影响因子：
6.2
通讯作者：
Chen, Jin
中科院分区：
文献类型：
--
作者：
Xu, Zhihui;Cai, Ling;Chen, Jin
在过去的十年中，人们对介孔二氧化硅材料作为药物控释载体进行了大量的研究，其中复杂的后合成过程往往涉及到分子设计以达到药物控释的目的。本研究以二茂铁羧酸（FCA）修饰的介孔SBA-15为载体，构建了一种抗癌药物阿霉素（DOX）的简化给药系统。通过物理化学表征的组合，锚定在胺基接枝的SBA-15的孔壁内的FCA的存在表现出电子可接近的行为，而不影响复合材料的完整性。在介孔复合材料的孔道中，DOX与FCA的结合实现了药物分子的pH响应性释放，这也有利于DOX的更大覆盖率。此外，进行实时细胞分析以监测DOX从中孔的释放，并评价所得的A549癌细胞的细胞毒性，其导致计算的IC 50为43.8 μ g/ml（24小时）。所构建的介孔FCA-SBA-15复合材料提供了一个集成的纳米平台，以发挥抗癌药物分子的控制递送。(C)2018爱思唯尔B. V.保留所有权利。
During the past decade, accumulating studies have been conducted on the mesoporous silica materials as the matrix of controlled drug-delivery, in which the complicated post-synthesis procedures are often involved with the molecular design to achieve the efficacy. In this study, a simplified drug delivery system of anti-cancer drug of doxorubicin (DOX) based on mesoporous SBA-15 functionalized with ferrocenecarboxylic acid (FCA) was constructed. Through a combination of physicochemical characterizations, the presence of FCA that was anchored inside the pore wall of amine groups grafted SBA-15 exhibits electron-accessible behavior without affecting the intactness of composite material. The pH-responsive release of drug molecules was achieved through the conjugation of DOX with FCA in the interior channels of mesoporous composites, which also favors the more coverage of DOX. Furthermore, the real-time cell analysis was performed to monitor the release of DOX from the mesopores and resulting cytotoxicity of cancer cell of A549 was evaluated, which results in a calculated IC50 of 43.8 mu g/ml (24 h). The constructed mesoporous FCA-SBA-15 composite material provides an integrated nanoplatform to exert controlled-delivery of anti-cancer drug molecules. (C) 2018 Elsevier B.V. All rights reserved.