Quantitative succinate dehydrogenase histochemistry in the hippocampus of aged rats
Quantitative succinate dehydrogenase histochemistry in the hippocampus of aged rats
复制标题
老年大鼠海马琥珀酸脱氢酶的定量组织化学
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Gehm
中科院分区：
文献类型：
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作者：
P. Kugler;S. Vogel;M. Gehm
摘要采用定量组织化学方法（显微光度动力学和终点测量，以及反应区域的形态计量分析）来研究年轻成年（3-6 月龄）和老年雄性大鼠（24-27 月龄）海马中琥珀酸脱氢酶（SDH）的水平。使用幼年动物海马体进行的有关 SDH 活性证明的方法学研究揭示了反应时间和反应产物量之间的线性关系长达 20 分钟；动力学（连续）和终点测量提供了相同的结果。在大量实验中，确定了由 100 mM 琥珀酸盐、10 mM 叠氮化钠、3 mM 氯化硝基蓝四唑、0.25 mM 吩嗪硫酸甲酯和 7.5% 聚乙烯醇组成的 0.05 M Hepes 缓冲液（最终 pH 7.5）组成的培养介质最适合海马中定量 SDH 组织化学。对大鼠海马 SDH 活性的比较定量研究表明，在年轻和老年大鼠海马的大部分区域和层中，SDH 活性水平沿海马头尾轴增加，即尾侧的水平高于头极。在两组中，最高的 SDH 水平出现在角 (CA)-1、CA-3 和齿状筋膜（中三分之一和外三分之一）的分子层，其中大部分是源自内嗅区的兴奋性穿通路径的终止区。此外，在几乎所有研究的蛋鸡中，老年动物的 SDH 水平低于年轻动物。这些差异在齿状筋膜的分子层和 CA-3 的大多数层中具有统计学意义。老年动物 SDH 水平较低与衰老大脑能量代谢能力降低有关。
SummaryQuantitative histochemical methods (microphotometric kinetic and end-point measurements, and morphometric analyses of reactive areas) were used to investigate the levels of succinate dehydrogenase (SDH) in the hippocampus of young adult (3–6 months old) and aged male rats (24–27 months old). Methodological studies concerning the demonstration of SDH activity, which were performed using hippocampi of young animals, revealed a linear relationship between the reaction time and the amount of reaction product for up to 20 min; kinetic (continuous) and end-point measurements provided the same results. In a number of experiments, it was established that an incubation medium consisting of 100 mM succinate, 10 mM sodium azide, 3 mM nitro blue tetrazolium chloride, 0.25 mM phenazine methosulfate, and 7.5% polyvinylalcohol in 0.05M Hepes buffer (final pH 7.5) was optimal for quantitative SDH histochemistry in the hippocampus. Comparative quantitative investigations of SDH activity in rat hippocampi showed that, in most regions and layers of the hippocampus of both young and aged rats, the levels of SDH activity increased along the rostrocaudal axis of the hippocampus, i.e., higher levels were present in the caudal than in the rostral pole. In both groups, the highest SDH levels were observed in the molecular layer of the cornu ammonis (CA)-1 the CA-3, and the fascia dentata (middle and outer thirds), most of which are termination fields of the excitatory perforant path arising from the regio entorhinalis. Furthermore, in almost all of the investigated layers, the older animals exhibited lower SDH levels than young animals. These differences were statistically significant in the molecular layer of the fascia dentata and in most layers of the CA-3. The lower SDH levels in aged animals are discussed in relation to the reduced capacity for energy metabolism in the aging brain.
衰老引起的脑氧化代谢异常及其与中枢去甲肾上腺素能系统的关系。
影响因子：
3.5
作者：
Sylvia,AL;Harik,SI;LaManna,JC;Wilkerson,T;Rosenthal,M
通讯作者：
Rosenthal,M