Expression of BAFF and BR3 in patients with systemic lupus erythematosus
Expression of BAFF and BR3 in patients with systemic lupus erythematosus
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DOI：
10.1590/1414-431x20154853
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发表时间：
2016-03-01
影响因子：
2.3
通讯作者：
Tang, Z.Q.
中科院分区：
文献类型：
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作者：
Duan, J.H.;Jiang, Y.;Tang, Z.Q.
本研究旨在探讨B细胞活化因子(BAFF)及其受体在系统性红斑狼疮(SLE)疾病活动性中的表达及其相互关系。采用实时荧光定量RT-PCR方法检测活动期和稳定期SLE患者及健康对照外周血单核细胞BAFF mRNA的表达。用流式细胞仪检测B淋巴细胞表面BAFF受体3(BR3)的百分率。采用双抗体夹心法测定血清中可溶性BAFF水平。用全自动免疫分析仪测定微量白蛋白水平。BAFF mRNA和可溶性BAFF水平在活动期SLE组最高，稳定期组次之，对照组最低(P
The objective of this study was to examine the relationship between the expression of B cell activating factor (BAFF) and BAFF receptor in patients with disease activity of systemic lupus erythematosus (SLE). Real-time RT-PCR was used to examine BAFF mRNA expression in peripheral blood monocytes of active and stable SLE patients and healthy controls. The percentage of BAFF receptor 3 (BR3) on B lymphocytes was measured by flow cytometry. Soluble BAFF levels in serum were assayed by ELISA. Microalbumin levels were assayed by an automatic immune analysis machine. BAFF mRNA and soluble BAFF levels were highest in the active SLE group, followed by the stable SLE group, and controls (P