Tobacco mosaic virus movement protein interacts with green fluorescent protein-tagged microtubule end-binding protein 1
Tobacco mosaic virus movement protein interacts with green fluorescent protein-tagged microtubule end-binding protein 1
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DOI：
10.1104/pp.108.117481
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发表时间：
2008-06-01
期刊：
影响因子：
7.4
通讯作者：
Heinlein, Manfred
中科院分区：
文献类型：
--
作者：
Brandner, Katrin;Sambade, Adrian;Heinlein, Manfred
烟草花叶病毒运动蛋白(MP)靶向胞间连丝涉及肌动蛋白/内质网网络，不需要完整的微管细胞骨架。然而，MP促进感染在细胞间传播的能力与该蛋白与微管的相互作用密切相关，表明微管系统参与了病毒RNA的运输。虽然MP的作用类似于微管相关蛋白，能够在感染后期稳定微管，但该蛋白也被证明在哺乳动物细胞中表达时导致中心体失活，从而表明MP可能与参与微管附着、成核或聚合的因素相互作用。为了进一步研究MP与植物微管系统的相互作用，我们在拟南芥绿色荧光蛋白(GFP)-融合微管末端结合蛋白1a(EB1a)的存在下表达了MP。这两种蛋白质在体内和体外共同定位和相互作用，并显示出相互的功能干扰。这些发现表明MP与EB1相互作用，这种相互作用可能在MP在感染过程中与微管系统的联系中发挥作用。
The targeting of the movement protein (MP) of Tobacco mosaic virus to plasmodesmata involves the actin/endoplasmic reticulum network and does not require an intact microtubule cytoskeleton. Nevertheless, the ability of MP to facilitate the cell-to-cell spread of infection is tightly correlated with interactions of the protein with microtubules, indicating that the microtubule system is involved in the transport of viral RNA. While the MP acts like a microtubule-associated protein able to stabilize microtubules during late infection stages, the protein was also shown to cause the inactivation of the centrosome upon expression in mammalian cells, thus suggesting that MP may interact with factors involved in microtubule attachment, nucleation, or polymerization. To further investigate the interactions of MP with the microtubule system in planta, we expressed the MP in the presence of green fluorescent protein (GFP)-fused microtubule end-binding protein 1a (EB1a) of Arabidopsis (Arabidopsis thaliana; AtEB1a: GFP). The two proteins colocalize and interact in vivo as well as in vitro and exhibit mutual functional interference. These findings suggest that MP interacts with EB1 and that this interaction may play a role in the associations of MP with the microtubule system during infection.