Role of fibronectin in the prevention of adherence of Pseudomonas aeruginosa to buccal cells.
Role of fibronectin in the prevention of adherence of Pseudomonas aeruginosa to buccal cells.
复制标题
纤连蛋白在防止铜绿假单胞菌粘附到口腔细胞中的作用。
DOI：
10.1093/infdis/143.6.784
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Bass,JA
中科院分区：
文献类型：
--
作者：
Woods,DE;Straus,DC;JohansonJr,WG;Bass,JA
最近的证据表明，革兰氏阴性杆菌在上呼吸道的定植是通过粘附于局部上皮细胞介导的。从12名重症患者的颊上皮细胞中获得用于研究，所有患者均被铜绿假单胞菌定殖。与来自未定殖对照的细胞相比，从这些患者获得的细胞粘附显著更多的P。在体外孵育期间的产气微生物。尽管定植患者细胞的唾液酸含量低于对照细胞，但用神经氨酸酶从正常细胞中去除唾液酸并不增加杆菌粘附。正常细胞的胰蛋白酶化增加了杆菌粘附，并显着减少了细胞表面纤维连接蛋白的量。胰蛋白酶消化的正常细胞和从重病患者中回收的细胞都附着大量的P。在体外培养的产气荚膜梭菌中，通过免疫荧光染色显示细胞表面的纤连蛋白减少。这些结果表明，宿主的改变与增加的易感性粘附由P。缺氧是纤维连接蛋白从细胞表面的损失。
Recent evidence suggests that colonization of the upper respiratory tract by gramnegative bacilli is mediated by adherence to regional epithelial cells. Buccal epithelial cells were obtained for study from 12 seriously ill patients, all of whom were colonized withPseudomonas aeruginosa. In comparison to cells from uncolonized controls, cells obtained from these patients attached significantly moreP. aeruginosaorganisms during incubation in vitro. Although the sialic acid content of colonized patients' cells was less then that of controls' cells, removal of sialic acid from normal cells with neuraminidase did not increase bacillary adherence. Trypsinization of normal cells increased bacillary adherence and significantly reduced the amount of fibronectin on the cell surface. Both trypsinized normal cells and cells recovered from seriously ill colonized patients attached large numbers ofP. aeruginosaorganisms in vitro and demonstrated decreased fibronectin on the cell surface by immunofluorescent staining. These findings suggest that the host alteration associated with increased susceptibility to adherence byP. aeruginosais the loss of fibronectin from the cell surface.