Differential localization of non‐muscle myosin II isoforms and phosphorylated regulatory light chains in human MRC‐5 fibroblasts
Differential localization of non‐muscle myosin II isoforms and phosphorylated regulatory light chains in human MRC‐5 fibroblasts
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DOI：
10.1016/s0014-5793(01)03186-6
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发表时间：
2001-12
期刊：
影响因子：
3.5
通讯作者：
Takayuki Saitoh;S. Takemura;K. Ueda;H. Hosoya;M. Nagayama;H. Haga;K. Kawabata;Akihiko Yamagishi;Masayuki Takahashi
中科院分区：
文献类型：
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作者：
Takayuki Saitoh;S. Takemura;K. Ueda;H. Hosoya;M. Nagayama;H. Haga;K. Kawabata;Akihiko Yamagishi;Masayuki Takahashi
我们研究了运动和非运动MRC-5成纤维细胞中非肌肉肌球蛋白II亚型和单（丝氨酸19）和双磷酸化（丝氨酸19和苏氨酸18）调节轻链（rlc）的定位。在迁移细胞中，肌球蛋白IIA定位于板层，并贯穿整个后区。除末端外，肌球蛋白IIB与肌球蛋白IIA在后部区域共定位。在肌球蛋白IIA富集的限制性区域检测到双磷酸化的rlc。在非运动细胞中，肌球蛋白IIA在具有二磷酸化rlc的外周应激纤维中富集，而肌球蛋白IIB则不富集。我们的研究结果表明，肌球蛋白IIA可能被rlc的二磷酸化高度激活，并主要参与细胞迁移。
We investigated the localization of non-muscle myosin II isoforms and mono- (at serine 19) and diphosphorylated (at serine 19 and threonine 18) regulatory light chains (RLCs) in motile and non-motile MRC-5 fibroblasts. In migrating cells, myosin IIA localized to the lamella and throughout the posterior region. Myosin IIB colocalized with myosin IIA to the posterior region except at the very end. Diphosphorylated RLCs were detected in the restricted region where myosin IIA was enriched. In non-motile cells, myosin IIA was enriched in peripheral stress fibers with diphosphorylated RLCs, but myosin IIB was not. Our results suggest that myosin IIA may be highly activated by diphosphorylation of RLCs and primarily involved in cell migration.