An endoplasmic reticulum-derived structure that is induced under stress conditions in Arabidopsis
An endoplasmic reticulum-derived structure that is induced under stress conditions in Arabidopsis
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DOI：
10.1104/pp.009464
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发表时间：
2002-12-01
期刊：
影响因子：
7.4
通讯作者：
Hara-Nishimura, I
中科院分区：
文献类型：
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作者：
Matsushima, R;Hayashi, Y;Hara-Nishimura, I
内质网（endoplasmic reticulum， ER）是内质网衍生的一种特征性结构，是一种帮助植物细胞在各种胁迫条件下进行蛋白酶分选的系统。在转基因拟南芥植株中观察到荧光内质网小体，表达融合内质网保留信号的绿色荧光蛋白。内质网小体广泛分布于整个幼苗的表皮细胞中。莲座叶无ER小体。我们发现创伤应激诱导了莲座叶片中许多内质网小体的形成。茉莉酸甲酯（MeJA）是一种植物激素，参与防御昆虫的伤害和咀嚼。乙烯抑制内质网小体的诱导。电镜分析表明，MeJA处理后的非转基因莲座叶片诱导出典型的内质网小体。利用coil和etr1-4突变体植株进行的实验表明，ER小体的诱导与MeJA和乙烯的信号转导密切相关。这些结果表明，内质网小体的形成是植物细胞响应环境胁迫的一种新颖而独特的内膜系统。内质网的生物学功能可能与高等植物的防御系统有关。
The endoplasmic reticulum (ER) body is a characteristic structure derived from ER and is referred to as a proteinase-sorting system that assists the plant cell under various stress conditions. Fluorescent ER bodies were observed in transgenic plants of Arabidopsis expressing green fluorescent protein fused with an ER retention signal. ER bodies were widely distributed in the epidermal cells of whole seedlings. In contrast, rosette leaves had no ER bodies. We found that wound stress induced the formation of many ER bodies in rosette leaves. ER bodies were also induced by treatment with methyl jasmonate (MeJA), a plant hormone involved in the defense against wounding and chewing by insects. The induction of ER bodies was suppressed by ethylene. An electron microscopic analysis showed that typical ER bodies were induced in the non-transgenic rosette leaves treated with MeJA. An experiment using coil and etr1-4 mutant plants showed that the induction of ER bodies was strictly coupled with the signal transduction of MeJA and ethylene. These results suggested that the formation of ER bodies is a novel and unique type of endomembrane system in the response of plant cells to environmental stresses. It is possible that the biological function of ER bodies is related to defense systems in higher plants.