Au nanorods-TiO2 photonic crystal plasmonic-photonic hybrid sensor for label-free detection and identification of DNA molecules with single nucleotide polymorphisms
Au nanorods-TiO2 photonic crystal plasmonic-photonic hybrid sensor for label-free detection and identification of DNA molecules with single nucleotide polymorphisms
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Au纳米棒-TiO2光子晶体等离子体-光子混合传感器，用于无标记检测和识别具有单核苷酸多态性的DNA分子
DOI：
10.1016/j.snb.2022.131747
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Endo Tatsuro
中科院分区：
文献类型：
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作者：
Kawasaki Daiki;Yamada Hirotaka;Sueyoshi Kenji;Hisamoto Hideaki;Endo Tatsuro
用于 DNA 无标记检测的高灵敏度光学传感器必须具有高可用性和广泛适用性，才能实现医学和生物科学领域的进步。然而，当前的传感器并不能同时满足这些标准。在这里，我们开发了一种简单、经济高效且灵敏的等离子体-光子混合传感器，使用金纳米棒作为纳米天线，并使用光子晶体板（PCS）作为微腔。在本报告中，我们对具有阿尔茨海默病相关单核苷酸多态性的DNA分子进行了简单的显微检测和鉴定，这是早期诊断的重要指标。 PCS 采用纳米压印光刻技术制造；金纳米棒与 PCS 可控耦合。通过控制等离激元-光子耦合共振来对设备灵敏度进行实验评估，这是通过调整 PCS 耦合的 AuNR 量来实现的。还通过基于时间耦合模式理论（TCMT）的混合传感器的理论建模来研究灵敏度。基于TCMT的理论模型很好地解释了实验结果。在 DNA 超灵敏检测演示中，成功实现了单核苷酸错配 DNA 分子 (~1 pM) 的识别和多个 DNA 分子 (~1000) 的检测，检测限为 5.9 aM。因此，该传感器可能在 DNA 分析中具有广泛的应用。
Highly sensitive optical sensors for label-free detection of DNA must have high usability and wide applicability for achieving advances in the medical and biological science fields. However, current sensors do not simultaneously satisfy these criteria. Here, we developed a simple, cost-effective, and sensitive plasmonic–photonic hybrid sensor using gold nanorods as nanoantennae and a photonic crystal slab (PCS) as a microcavity. In this report, we performed simple microscopic detection and identification of DNA molecules with Alzheimer’s disease-associated single nucleotide polymorphisms, which is an important indicator for early diagnosis. The PCS was fabricated using nanoimprint lithography; the gold nanorods were controllably coupled with the PCS. The device sensitivity was experimentally assessed by controlling the plasmonic–photonic coupling resonance, which was enabled by tuning the PCS-coupled AuNR amounts. The sensitivity was also investigated by theoretical modeling of the hybrid sensor, based on the temporal coupled mode theory (TCMT). The TCMT-based theoretical model well explained the experimental results. In a demonstration of ultrasensitive detection of DNA, the identification of single nucleotide mismatched DNA molecules (~1 pM) and detection of several DNA molecules (~1000), with a limit of detection of 5.9 aM, were successfully achieved. This sensor may thus have wide applications for DNA analysis.