A Resequencing-Based Ultradense Genetic Map of Hericium erinaceus for Anchoring Genome Sequences and Identifying Genetic Loci Associated With Monokaryon Growth
A Resequencing-Based Ultradense Genetic Map of Hericium erinaceus for Anchoring Genome Sequences and Identifying Genetic Loci Associated With Monokaryon Growth
复制标题
基于重测序的猴头菇超密集遗传图谱，用于锚定基因组序列并识别与单核生长相关的遗传位点
DOI：
10.3389/fmicb.2019.03129
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发表时间：
2020-01-31
影响因子：
5.2
通讯作者：
Peng, Yuande
中科院分区：
文献类型：
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作者：
Gong, Wenbing;Xie, Chunliang;Peng, Yuande
猴头菌因其引人注目的健康促进特性而引起了人们的极大兴趣。然而，选育优良品种的H。猴头菌由于缺乏遗传和分子工具箱而受到阻碍。本研究对127个F1单孢菌株进行了重测序分析，构建了我国第一张高分辨率的H.刺猬。使用重组箱作为标记，生成了由1，174个箱（包括37，082个单核苷酸多态性）组成的超密集遗传图谱。这个新开发的遗传图谱覆盖了1，096.5 cM，平均箱间距为0.95 cM。遗传图谱与H.通过使用bin标记物作为锚将支架与该遗传图谱进行比对，揭示了猴头菌基因组组装。初步探讨了该遗传图谱在数量性状基因座（QTL）定位中的应用，并回收了4个影响单核体生长的QTL。其中一个QTL mgr 1位于交配型A（MAT-A）位点附近，对生长变异的贡献率为12.1%。总体而言，这张新构建的高分辨率遗传图谱（或bin图谱）可作为未来H.的遗传、基因组和育种研究的参考。刺猬。
Hericium erinaceus has attracted tremendous interest owing to its compelling health-promoting properties. However, breeding of elite cultivars of H. erinaceus is hindered by the lack of a genetic and molecular toolbox. Here, we performed resequencing analysis of 127 F1 single-spore isolates and constructed the first high-resolution genetic map of H. erinaceus. With the use of recombination bins as markers, an ultradense genetic map consisting of 1,174 bins (including 37,082 single-nucleotide polymorphisms) was generated. This newly developed genetic map covered 1,096.5 cM, with an average bin spacing of 0.95 cM. High collinearity between genetic map and H. erinaceus genome assembly was revealed by aligning scaffolds to this genetic map using bin markers as anchors. The application of this newly developed genetic map in quantitative trait locus (QTL) mapping was also elucidated, and four QTLs for monokaryon growth were recovered. One QTL, mgr1, which contributes 12.1% of growth variations, was located near the mating type A (MAT-A) loci. Overall, this newly constructed high-resolution genetic map (or bin map) could be used as reference in future genetic, genomic, and breeding studies on H. erinaceus.