Quantifying CFSE label decay in flow cytometry data
Quantifying CFSE label decay in flow cytometry data
复制标题
DOI：
10.1016/j.aml.2012.12.010
复制
发表时间：
2013-05-01
影响因子：
3.7
通讯作者：
Thompson, W. C.
中科院分区：
文献类型：
--
作者：
Banks, H. T.;Choi, A.;Thompson, W. C.
我们开发了一系列模型的细胞增殖试验中的标记衰减时，细胞内染料羧基荧光素琥珀酰亚胺酯（CFSE）被用作染色剂。从两名健康患者收集的数据被用来验证模型，并比较模型与Akiake信息标准。在数据中的多个衰减率的显着特点很容易通过时间依赖的衰减模型，如Logistic和Gompertz模型的特点和解释。(C)2013爱思唯尔有限公司保留所有权利。
We developed a series of models for the label decay in cell proliferation assays when the intracellular dye carboxyfluorescein succinimidyl ester (CFSE) is used as a staining agent. Data collected from two healthy patients were used to validate the models and to compare the models with the Akiake Information Criteria. The distinguishing features of multiple decay rates in the data are readily characterized and explained via time dependent decay models such as the logistic and Gompertz models. (C) 2013 Elsevier Ltd. All rights reserved.