Decreased dendritic spine density on prefrontal cortical and hippocampal pyramidal neurons in postweaning social isolation rats
Decreased dendritic spine density on prefrontal cortical and hippocampal pyramidal neurons in postweaning social isolation rats
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DOI：
10.1016/s0006-8993(03)03042-7
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发表时间：
2003-09-05
期刊：
影响因子：
2.9
通讯作者：
Flores, G
中科院分区：
文献类型：
--
作者：
Silva-Gómez, AB;Rojas, D;Flores, G
本实验观察了断奶后社会隔离（Postweaning social isolation，CSSI）对大鼠前额叶皮质内侧部（medial part of prefrontal cortex，mPFC）和海马锥体神经元形态学的影响。动物在出生后第21天（P21）断奶并隔离8周。隔离期后，在自发活动室中评价60 min内的自发活动，并通过过量戊巴比妥钠处死动物，并心内灌注0.9%盐水溶液。取出脑，通过Golgi-Cox染色处理并通过Sholl方法分析。与对照组相比，隔离大鼠在新环境中的运动活动增加。树突状形态学清楚地表明，在mPFC的锥体神经元中没有变化的情况下，CA 1的Carnpus锥体细胞的树突长度减少。而mPFC和海马锥体细胞树突棘密度减少。此外，Sholl分析表明，与对照组相比，仅在Escarnpus区域，EscarnSI产生了Sholl交叉点数量的减少。目前的结果表明，PDSI可能部分影响边缘系统结构，如mPFC和海马，在精神分裂症有牵连的树突形态。(C)2003 Elsevier B. V.保留所有权利。
The effects of postweaning social isolation (pwSI) on the morphology of the pyramidal neurons from the medial part of the prefrontal cortex (mPFC) and hippocampus were investigated in rats. The animals were weaned on day 21 postnatal (P21) and isolated 8 weeks. After the isolation period, locomotor activity was evaluated through 60 min in the locomotor activity chambers and the animals were sacrificed by overdoses of sodium pentobarbital and perfused intracardially with 0.9% saline solution. The brains were removed, processed by the Golgi-Cox stain and analyzed by the Sholl method. The locomotor activity in the novel environment from the isolated rats was increased with respect to the controls. The dendritic morphology clearly showed that the pwSI animals presented a decrease in dendritic length of pyramidal cells from the CA1 of the hippocarnpus without changes in the pyramidal neurons of the mPFC. However, the density of dendritic spines was decreased in the pyramidal cells from mPFC and Hippocampus. In addition, the Sholl analyses showed that pwSI produced a decrease in the number of sholl intersections compared with the control group only in the hippocarnpus region. The present results suggest that pwSI may in part affect the dendritic morphology in the limbic structures such as mPFC and hippocampus that are implicated in schizophrenia. (C) 2003 Elsevier B.V. All rights reserved.