Quantitative trait loci for maternal performance for offspring survival in mice.
Quantitative trait loci for maternal performance for offspring survival in mice.
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发表时间：
2002-11
期刊：
影响因子：
3.3
通讯作者：
A. Peripato;R. A. de Brito;T. Vaughn;L. S. Pletscher;S. R. Matioli;J. Cheverud
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文献类型：
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作者：
A. Peripato;R. A. de Brito;T. Vaughn;L. S. Pletscher;S. R. Matioli;J. Cheverud
母亲的表现是指母亲所提供的环境对其后代的表型，如后代的生存和生长所产生的影响。母亲行为和生理的变化导致母亲表现的变化，进而影响后代的生存。在我们的研究中，我们发现雌性不能养育后代，表现出异常的母性行为。以SM/J和LG/J自交系F(2)杂交的241只雌性小鼠为研究对象，研究了母性表现对后代生存的遗传结构。利用区间定位方法，我们在2号染色体和7号染色体上分别发现了影响母系D2Mit17 + 6cm和D7Mit21 + 2cm的两个数量性状位点（QTL）。在双向全基因组上位性扫描中，我们发现了15个上位性相互作用，涉及23个QTL，分布在除12、16和17染色体外的所有染色体上。这些位点形成了几组相互作用的QTL，表明一套复杂的机制决定了母亲对后代生存的表现。综合考虑并校正了大量的重要因素，QTL及其相互作用解释了该杂交中近35%的母体表现对后代存活的表型变异。这项研究可以确定许多可能的候选基因，以及影响小鼠母性表现的基因效应的相对大小和基因作用模式。对后代母亲的详细行为观察表明，第一周的后代存活率与母亲在筑巢、梳理幼崽、提供乳汁和/或表现出对入侵者的攻击行为方面的成功有关。
Maternal performance refers to the effect that the environment provided by mothers has on their offspring's phenotypes, such as offspring survival and growth. Variations in maternal behavior and physiology are responsible for variations in maternal performance, which in turn affects offspring survival. In our study we found females that failed to nurture their offspring and showed abnormal maternal behaviors. The genetic architecture of maternal performance for offspring survival was investigated in 241 females of an F(2) intercross of the SM/J and LG/J inbred mouse strains. Using interval-mapping methods we found two quantitative trait loci (QTL) affecting maternal performance at D2Mit17 + 6 cM and D7Mit21 + 2 cM on chromosomes 2 and 7, respectively. In a two-way genome-wide epistasis scan we found 15 epistatic interactions involving 23 QTL distributed across all chromosomes except 12, 16, and 17. These loci form several small sets of interacting QTL, suggesting a complex set of mechanisms operating to determine maternal performance for offspring survival. Taken all together and correcting for the large number of significant factors, QTL and their interactions explain almost 35% of the phenotypic variation for maternal performance for offspring survival in this cross. This study allowed the identification of many possible candidate genes, as well as the relative size of gene effects and patterns of gene action affecting maternal performance in mice. Detailed behavior observation of mothers from later generations suggests that offspring survival in the first week is related to maternal success in building nests, grooming their pups, providing milk, and/or manifesting aggressive behavior against intruders.