Regulation of CART mRNA by stress and corticosteroids in the hippocampus and amygdala
Regulation of CART mRNA by stress and corticosteroids in the hippocampus and amygdala
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DOI：
10.1016/j.brainres.2007.03.042
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发表时间：
2007-06-04
期刊：
影响因子：
2.9
通讯作者：
McEwen, Bruce S.
中科院分区：
文献类型：
--
作者：
Hunter, Richard G.;Bellani, Rudy;McEwen, Bruce S.
CART（可卡因-安非他明调节转录本）已被证明是由皮质类固醇在下丘脑，但其调节皮质类固醇和应激尚未得到很好的检查，在海马或杏仁核。此外，CART与青春期的过渡有关。在这项研究中，我们研究了急性（30分钟）应激对CART mRNA在青春期前和成年大鼠的影响。此外，我们还研究了慢性（21天× 6小时）束缚应激对海马和杏仁核中CART mRNA表达的影响，以及7天肾上腺切除术和皮质类固醇替代对成年大鼠大脑这些区域中CART表达的影响。我们发现CART mRNA在中央杏仁核的急性而非慢性应激诱导的上调和慢性而非急性应激诱导的齿状回的上调。肾上腺切除术减少CART表达的齿状回，但不是杏仁核，这种效果被皮质酮，但不是RU 28，362或醛固酮替代阻断，这表明盐皮质激素和糖皮质激素受体的协同作用。我们的数据表明，CART的表达是由应激在一个区域和时间特异性的方式进行调节，CART是由皮质类固醇在海马中的作用。(c)2007 Elsevier B. V.保留所有权利。
CART (Cocaine-Amphetamine-Regulated Transcript) has been shown to be regulated by corticosteroids in the hypothalamus, but its regulation by corticosteroids and stress has not been well examined in the hippocampus or the amygdala. Further, CART has been implicated in the transition to puberty. In this study we examine the effects of acute (30 min) stress on CART mRNA in prepubescent and adult rats. In addition, we examined chronic (21 day x 6 h) restraint stress upon the expression of CART mRNA in the hippocampus and the amygdala and the effects of 7 days of adrenalectomy and corticosteroid replacement upon CART expression in these regions of the adult rat brain. We found an up-regulation of CART mRNA in the central amygdala induced by acute but not chronic stress and an upregulation in the dentate gyrus induced by chronic but not acute stress. Adrenalectomy reduced CART expression in the dentate gyrus but not the amygdala and this effect was blocked by corticosterone but not RU28,362 or aldosterone replacement, suggesting a synergism of mineralocorticoid and glucocorticoid receptors. Our data establish that CART expression is regulated by stress in a regionally and time specific manner and that CART is regulated by corticosteroid actions in the hippocampus. (c) 2007 Elsevier B.V. All rights reserved.