MODULATION OF TYPE-BETA TRANSFORMING GROWTH-FACTOR ACTIVITY IN BONE CULTURES BY OSTEOTROPIC HORMONES
MODULATION OF TYPE-BETA TRANSFORMING GROWTH-FACTOR ACTIVITY IN BONE CULTURES BY OSTEOTROPIC HORMONES
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DOI：
10.1073/pnas.84.7.2024
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
MUNDY, GR
中科院分区：
文献类型：
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作者：
PFEILSCHIFTER, J;MUNDY, GR
类型β。转化生长因子（TGF-β）通过使用正常大鼠肾成纤维细胞的锚定非依赖性生长作为指示系统，在从胎鼠和新生小鼠头盖骨收获的条件培养基中发现了活性。与甲状旁腺激素、1，25-二羟基维生素D3和白细胞介素1（所有刺激骨吸收的因子）一起孵育的颅骨显示TGF-β的浓度依赖性增加。培养基中的活性。TGF-β的增加除去这些因素后，活性持续48小时不减弱。TGF-β的增加来自再吸收骨培养物的活性相对大于骨再吸收的增加。降钙素对骨吸收的抑制与TGF-β的降低相关。活动因此，调节骨吸收的试剂也影响TGF-β。骨培养基中的活性。TGF-β局部浓度的变化由促骨激素引起的活性在正常骨重建的调节中可能是重要的。
Type .beta. transforming growth factor (TGF-.beta.) activity was found in conditioned medium harvested from fetal rat and neonatal mouse calvariae by using the anchorage-independent growth of normal rat kidney fibroblasts as an indicator system. Calvariae incubated with parathyroid hormone, 1,25-dihydroxyvitamin D3, and interleukin 1, all factors that stimulate bone resorption, showed a concentration-dependent increase in TGF-.beta. activity in the culture medium. Increases in TGF-.beta. activity persisted undiminished for 48 hr after removal of these factors. The increases in TGF-.beta. activity from the resorbing bone cultures were relatively greater than the increases in bone resorption. Calcitonin inhibition of bone resorption correlated with a decrease in TGF-.beta. activity. Thus, agents that modulate bone resorption also effect TGF-.beta. activity in the bone culture medium. Changes in local concentrations of TGF-.beta. activity by osteotropic hormones may be important in the regulation of normal bone remodeling.