Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells
Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells
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DOI：
10.1038/s41551-018-0200-7
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发表时间：
2018-03
影响因子：
28.1
通讯作者：
Shin-ya Yasuda;T. Ikeda;Hosein Shahsavarani;N. Yoshida;Bhavana Nayer;Motoki Hino;Neha Vartak-Sharma;H. Suemori;Kouichi Hasegawa
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文献类型：
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作者：
Shin-ya Yasuda;T. Ikeda;Hosein Shahsavarani;N. Yoshida;Bhavana Nayer;Motoki Hino;Neha Vartak-Sharma;H. Suemori;Kouichi Hasegawa
用于细胞治疗和药物发现的质量受控的人多能干细胞（hPSC）的大规模和成本有效的生产理想地需要化学定义的无异生物质的培养系统。为了开发这种系统，需要降低与使用重组蛋白作为基础培养基中的补充物相关的成本。在这里，我们描述了一种无生长因子的培养基，它只使用三种化合物和比市售培养基中使用的重组蛋白数量更少的重组蛋白。我们表明，培养基支持hPSC的长期繁殖，如通过核型、多能性标志物的表达和分化成源自三个胚胎胚层的细胞类型的能力所证实的。在培养基中生长的hPSC比在含有生长因子的培养基中生长的细胞更少地依赖于糖酵解途径。此外，该培养基支持从人真皮成纤维细胞或外周血单核细胞衍生的诱导多能干细胞的产生。我们的研究结果将促进hPSC完全无异种、化学定义的合成培养系统的持续开发。
The large-scale and cost-effective production of quality-controlled human pluripotent stem cells (hPSCs) for use in cell therapy and drug discovery would ideally require a chemically defined xenobiotic-free culture system. Towards the development of such a system, costs associated with the use of recombinant proteins as supplements in basal culture media need to be reduced. Here, we describe a growth-factor-free culture medium that uses just three chemical compounds and a lower number of recombinant proteins than used in commercially available media. We show that the culture medium supports the long-term propagation of hPSCs, as confirmed by karyotype, the expression of pluripotency markers and the capacity to differentiate into cell types derived from the three embryonic germ layers. hPSCs growing in the medium were less dependent on glycolytic pathways than cells grown in medium containing growth factors. Moreover, the medium supported the generation of induced pluripotent stem cells derived from either human dermal fibroblasts or peripheral blood mononuclear cells. Our findings should facilitate the ongoing development of a completely xeno-free, chemically defined, synthetic culture system for hPSCs.