Over-expression of β-1,4-galactosyltransferase I, II, and V in human astrocytoma
Over-expression of β-1,4-galactosyltransferase I, II, and V in human astrocytoma
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DOI：
10.1007/s004320100246
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发表时间：
2001-08
影响因子：
3.6
通讯作者：
Songli Xu;Xiaoyu Zhu;Songwen Zhang;S. Yin;Liangfu Zhou;C. Chen;J. Gu
中科院分区：
文献类型：
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作者：
Songli Xu;Xiaoyu Zhu;Songwen Zhang;S. Yin;Liangfu Zhou;C. Chen;J. Gu
目的：β-1，4-半乳糖基转移酶(β-1，4-GalT)I、II和V是通过将半乳糖转移到N-乙酰氨基葡萄糖末端形成β-1，4-键，在N-糖链上生物合成N-乙酰乳糖胺的酶。因此，我们比较了β-1，4-Galts在星形细胞瘤和正常脑组织中的表达。结果：我们的逆转录-聚合酶链式反应结果显示，β-1，4-GalT I在正常成人脑组织中不存在，但在II，III，IV级星形细胞瘤中可检测到，β-1，4-GalT II在II，III，IV级星形细胞瘤中表达增加，而在正常脑组织中仅有微量表达；β-1，4-GalT V转录本在正常脑组织中存在，并随着星形细胞瘤的进展而增加，在IV级星形细胞瘤中表达水平最高。通过蓖麻凝集素-1(RCA-1)凝集素印迹分析，我们还发现星形细胞瘤与正常脑组织相比有更广泛的半乳糖化条带。结论：星形细胞瘤中β-1，4-GalT I、II和V基因表达的改变可能是导致其半乳糖化水平升高的原因，β-1，4-GalT V的表达与星形细胞瘤的恶性程度有关。
Purpose:β-1,4-Galactosyltransferase (β-1,4-GalT) I, II, and V are the enzymes responsible for the biosynthesis of N-acetyllactosamine on N-glycans by transferring UDP-galactose to the terminal N-acetylglucosamine (N-GlcNAc) residues with the formation of a β-1,4-linkage. Neoplasms undergo various changes in the carbohydrate of their glycoconjugates, indicating the possible changes in glycosyltransferases themselves.Method:Therefore, we compared the expression of β-1,4-GalTs between astrocytoma and normal brain tissues.Results:Our reverse-transcription polymerase chain reaction (RT-PCR) results showed that β-1,4-GalT I transcript was absent in normal adult brain but detectable in grade II, III, and IV astrocytomas; the level of β-1,4-GalT II transcript was increased in grade II, III, and IV astrocytomas while only a trace amount was found in normal brain; β-1,4-GalT V transcript existed in normal brain and increased in the process of astrocytoma progress, with the highest level in grade IV astrocytoma. ByRicinus communisagglutinin-1 (RCA-1) lectin blot assay, we also found the more extensive galactosylated bands in astrocytomas compared with normal brain. A major 61kD protein was galactosylated in astrocytoma but not in normal brain tissues.Conclusion:These results indicate that the increase of galactosylation in astrocytomas may be caused by the alterations of gene expression of β-1,4-GalT I, II, and V and that the malignant degree of astrocytoma is correlated with the expression of β-1,4-GalT V.