Leukotrienes mediate neurogenic inflammation in lungs of young rats infected with respiratory syncytial virus.
Leukotrienes mediate neurogenic inflammation in lungs of young rats infected with respiratory syncytial virus.
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白三烯介导感染呼吸道合胞病毒的幼鼠肺部神经源性炎症。
DOI：
10.1152/ajplung.00323.2001
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Piedimonte,Giovanni
中科院分区：
文献类型：
--
作者：
Wedde-Beer,Katrin;Hu,Chengping;Rodriguez,MariaM;Piedimonte,Giovanni
呼吸道合胞病毒（RSV）感染增强大鼠气道神经源性炎症。由于感觉神经的一些血管效应是由半胱氨酰白三烯（cysLTs）介导的，我们研究了受体拮抗剂孟鲁司特是否抑制RSV感染大鼠的神经源性血浆外渗。无病原体大鼠在2周龄（断奶）或12周龄（成年）时接种RSV或无病毒培养基，并在接种前1天开始用孟鲁司特或其溶媒处理。接种后5天，我们测量了用辣椒素刺激感觉神经后呼吸道中伊文思蓝标记的白蛋白的外渗。孟鲁司特对肺外气道没有影响，但能消除RSV感染大鼠肺内气道中的白蛋白外渗，对断奶大鼠的影响大于成年大鼠。在RSV感染的断奶大鼠的肺组织中检测到5-脂氧合酶编码mRNA和cysLTs以及众多肥大细胞浓度增加。这些观察结果表明，从辣椒素敏感的感觉神经和非神经元细胞在RSV感染的年轻大鼠肺神经肽的释放增加血管通透性，促进释放白三烯肥大细胞。
Respiratory syncytial virus (RSV) infection potentiates neurogenic inflammation in rat airways. Because some vascular effects of sensory nerves are mediated by cysteinyl leukotrienes (cysLTs), we studied whether the receptor antagonist montelukast inhibits neurogenic plasma extravasation in RSV-infected rats. Pathogen-free rats were inoculated at 2 wk (weanlings) or 12 wk (adults) of age with RSV or virus-free medium and treated with montelukast or its vehicle starting 1 day before inoculation. Five days postinoculation, we measured the extravasation of Evans blue-labeled albumin in the respiratory tract after stimulation of sensory nerves with capsaicin. Montelukast had no effect in the extrapulmonary airways but abolished albumin extravasation in the intrapulmonary airways of RSV-infected rats, with a larger effect in weanlings than in adults. Increased concentrations of 5-lipoxygenase-encoding mRNA and cysLTs, as well as numerous mast cells, were detected in the lung tissues of RSV-infected weanling rats. These observations suggest that the release of neuropeptides from capsaicin-sensitive sensory nerves and nonneuronal cells in the lungs of RSV-infected young rats increases vascular permeability by promoting the release of leukotrienes from mast cells.