EVIDENCE FOR A HIGH AND SPECIFIC CONCENTRATION OF (NA+,K+)ATPASE IN THE PLASMA-MEMBRANE OF THE OSTEOCLAST
EVIDENCE FOR A HIGH AND SPECIFIC CONCENTRATION OF (NA+,K+)ATPASE IN THE PLASMA-MEMBRANE OF THE OSTEOCLAST
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DOI：
10.1016/0092-8674(86)90748-8
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发表时间：
1986-07-18
期刊：
影响因子：
64.5
通讯作者：
CAPLAN, M
中科院分区：
文献类型：
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作者：
BARON, R;NEFF, L;CAPLAN, M
在骨吸收过程中，破骨细胞主动酸化有限的细胞外腔。我们推测，像其他参与离子转运和质子转运的细胞一样，破骨细胞的S膜可能在钠泵中高度浓缩。使用针对α-抗体的单抗。还有测试版。通过亚基、免疫印迹分析和[~3H]哇巴因结合实验，我们证明了破骨细胞质膜与其他骨细胞、单核细胞、巨噬细胞以及其他血液和骨髓细胞相比，具有高度和特异性的(Na+，K+)ATPase富集性。破骨细胞上结合部位的密度与肾小管细胞相当。这一观察结果与(Na+，K+)ATPase在骨吸收机制中发挥作用的假说一致，可能与次级活性钙和/或质子转运有关。因此，抗Na+，K+ATPase的单抗可作为破骨细胞在骨和骨髓制剂中的特异性标志物。
During bone resorption, the osteoclast actively acidifies a limited extracellular compartment. We hypothesized that, like other cells engaged in ion transport and proton translocation, the osteoclast''s membrane might be highly enriched in sodium pumps. Using monoclonal antibodies to both the .alpha. and the .beta. subunits, immunoblot analysis, and [3H]ouabain binding, we have demonstrated that the osteoclast plasma membrane is both highly and specifically enriched in (Na+, K+)ATPase, compared with other bone cells, monocytes, macrophages, and other blood and bone marrow cells. The density of binding sites on the osteoclast is equivalent to that of kidney tubule cells. This observation is consistent with the hypothesis that the (Na+,K+)ATPase plays a role in the mechanism of bone resorption, possibly coupled with secondary active calcium and/or proton transport. Monoclonal antibodies against the Na+,K+ATPase can therefore be used as speecific markers for the osteoclast in bone and bone marrow preparations.