Dietary whey hydrolysate with exercise alters the plasma protein profile: A comprehensive protein analysis
Dietary whey hydrolysate with exercise alters the plasma protein profile: A comprehensive protein analysis
复制标题
DOI：
10.1016/j.nut.2010.06.004
复制
发表时间：
2011-06-01
期刊：
影响因子：
4.4
通讯作者：
Yoshikawa, Toshikazu
中科院分区：
文献类型：
--
作者：
Aoi, Wataru;Takanami, Yoshikazu;Yoshikawa, Toshikazu
目的：研究表明，膳食乳清蛋白通过改变骨骼肌中的糖调节酶活性来加速葡萄糖的摄取。在本研究中，我们研究了膳食乳清蛋白对耐力和糖原再合成的影响，并试图通过综合蛋白质组学方法鉴定反映身体状况的血浆蛋白。方法：将雄性c57BL/6小鼠分为四组：久坐组、久坐乳清蛋白水解物组、运动组和乳清蛋白水解物运动组。运动组的小鼠每周进行五次跑步机跑步运动，持续 4 周。对从个体获得的血浆样本进行蛋白质分析，并测量耐力表现和腓肠肌糖原含量。结果：训练期结束后，与喂养对照饮食的小鼠相比，喂养乳清饮食的小鼠的耐力得到改善。运动4周后肌糖原含量显着增加，乳清蛋白的摄入导致糖原进一步增加。研究发现载脂蛋白 A-II 和 C-I 以及 β(2)-糖蛋白-1 通过训练与乳清蛋白摄入相结合而发生改变，而运动或单独乳清蛋白不会引起显着变化。结论：本研究的结果表明，这三种蛋白可能是改善耐力和糖原再合成的潜在生物标志物，也是介导乳清益处的部分机制 蛋白质。 (C) 2011 Elsevier Inc. 保留所有权利。
Objective: It has been shown that dietary whey protein accelerates glucose uptake by altering glycoregulatory enzyme activity in skeletal muscle. In the present study, we investigated the effect of dietary whey protein on endurance and glycogen resynthesis and attempted to identify plasma proteins that reflected the physical condition by a comprehensive proteomics approach.Methods: Male c57BL/6 mice were divided into four groups: sedentary, sedentary with whey protein hydrolysate, exercise, and exercise with whey protein hydrolysate. The mice in the exercise groups performed treadmill running exercise five times per week for 4 wk. Protein profiling of plasma sample obtained from individuals was performed, as were measurements of endurance performance and the glycogen content of gastrocnemius muscle.Results: After the training period, the endurance of mice fed the whey diet was improved compared with that of mice fed the control diet. Muscle glycogen content was significantly increased after 4 wk of exercise, and intake of whey protein led to a further increase in glycogen. Apolipoproteins A-II and C-I and beta(2)-glycoprotein-1 were found to be altered by training combined with the intake of whey protein, without significant changes induced by exercise or whey protein alone.Conclusion: Results of the present study suggest that these three proteins may be potential biomarkers of improved endurance and glycogen resynthesis and part of the mechanism that mediates the benefits of whey protein. (C) 2011 Elsevier Inc. All rights reserved.