The WFDC1 gene encoding ps20 localizes to 16q24, a region of LOH in multiple cancers
The WFDC1 gene encoding ps20 localizes to 16q24, a region of LOH in multiple cancers
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DOI：
10.1007/s003350010135
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发表时间：
2000-09-01
期刊：
影响因子：
2.5
通讯作者：
Rowley, DR
中科院分区：
文献类型：
--
作者：
Larsen, M;Ressler, SJ;Rowley, DR
我们先前鉴定了一种分泌型生长抑制蛋白，并从胎鼠前列腺、泌尿生殖窦间充质细胞条件培养液中纯化了该蛋白。随后克隆了大鼠的cDNA，并发现ps20含有一个WAP类型的四个二硫键核心基序，这表明它可能是一种蛋白酶抑制因子。我们现在报道了小鼠的ps20基因(命名为Wfdc1)、人的同源基因cDNA和人类的基因(命名为wfdc1)的克隆和鉴定，鼠和人的wfdc1基因都由7个外显子组成，编码各自的ps20蛋白，在外显子2中有79.1%的同源性和几乎相同的WAP基序。通过FISH分析，WFDc1基因被定位到人类染色体(Chr)16q24，这是一个以前在前列腺癌、乳腺癌、肝细胞癌和Wilms‘Turner等多种癌症中发现的频繁杂合性缺失(LOH)区域。WFDC1基因的鉴定和鉴定可能有助于更好地理解该基因和ps20在前列腺生物学和癌症发生中的潜在作用。
We previously identified ps20 protein as a secreted growth inhibitor and purified the protein from fetal rat prostate urogenital sinus mesenchymal cell conditioned medium. The rat cDNA was subsequently cloned, and ps20 was found to contain a WAP-type four-disulfide core motif, indicating it may function as a protease inhibitor. We now report cloning and characterization of the mouse ps20 gene (designated Wfdc1), the human homolog cDNA, and the human gene (designated WFDC1), Both the mouse and human WFDC1 genes consist of seven exons and encode respective ps20 proteins sharing 79.1% identity and nearly identical WAP motifs in exon 2. The WFDC1 gene was mapped by FISH analysis to human Chromosome (Chr) 16q24, an area of frequent loss of heterozygosity (LOH) previously identified in multiple cancers including prostate, breast, hepatocellular, and Wilms' turner. identification and characterization of the WFDC1 gene may aid in better understanding the potential role of this gene and ps20 in prostate biology and carcinogenesis.