Hoxb1 functions in both motoneurons and in tissues of the periphery to establish and maintain the proper neuronal circuitry
Hoxb1 functions in both motoneurons and in tissues of the periphery to establish and maintain the proper neuronal circuitry
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DOI：
10.1101/gad.1207204
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发表时间：
2004-07-01
影响因子：
10.5
通讯作者：
Capecchi, MR
中科院分区：
文献类型：
--
作者：
Arenkiel, BR;Tvrdik, P;Capecchi, MR
头部神经回路的形成需要中枢神经系统（CNS）和神经嵴衍生的外周靶组织内神经元的协调发育。Hoxb1，这是整个菱形4（r4）的表达，已被证明是所需的面鳃神经元祖细胞的编程神经支配面部表情的肌肉的规格。在这项研究中，我们发现了Hoxb1表达细胞在第七脑神经回路的形成和维持中的额外作用。通过有条件地删除神经嵴中的Hoxb1位点，我们证明了R4衍生的神经嵴中也需要Hoxb1来促进和维持第VII神经回路的形成。遗传谱系分析表明，一个重要的人口r4衍生的神经嵴是注定要产生神经胶质髓鞘的第七颅神经。神经嵴文化表明，Hoxb1功能的缺乏似乎不会影响整体胶质祖细胞的规格，这表明，后来的胶质功能是至关重要的第七神经的维护。两者合计，这些结果表明，第七脑神经的发展和维护的分子程序依赖于Hoxb1，无论是在神经嵴衍生的胶质细胞和面鳃神经元。
Formation of neuronal circuits in the head requires the coordinated development of neurons within the central nervous system (CNS) and neural crest-derived peripheral target tissues. Hoxb1, which is expressed throughout rhombomere 4 (r4), has been shown to be required for the specification of facial branchiomotor neuron progenitors that are programmed to innervate the muscles of facial expression. In this study, we have uncovered additional roles for Hoxb1-expressing cells in the formation and maintenance of the VIIth cranial nerve circuitry. By conditionally deleting the Hoxb1 locus in neural crest, we demonstrate that Hoxb1 is also required in r4-derived neural crest to facilitate and maintain formation of the VIIth nerve circuitry. Genetic lineage analysis revealed that a significant population of r4-derived neural crest is fated to generate glia that myelinate the VIIth cranial nerve. Neural crest cultures show that the absence of Hoxb1 function does not appear to affect overall glial progenitor specification, suggesting that a later glial function is critical for maintenance of the VIIth nerve. Taken together, these results suggest that the molecular program governing the development and maintenance of the VIIth cranial nerve is dependent upon Hoxb1, both in the neural crest-derived glia and in the facial branchiomotor neurons.