Growth-related expression of a 72,000 molecular weight poly(A)+ mRNA binding protein.
Growth-related expression of a 72,000 molecular weight poly(A)+ mRNA binding protein.
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72,000 分子量的 Poly(A) mRNA 结合蛋白的生长相关表达。
DOI：
10.1016/0014-4827(88)90398-9
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发表时间：
1988
影响因子：
3.7
通讯作者：
Mercer,WE
中科院分区：
文献类型：
--
作者：
Ullrich,SJ;Appella,E;Mercer,WE
在这次交流中，我们研究了一个72000molw(P72)的宿主蛋白，它与针对猿猴病毒40(SV40)大T抗原蛋白上的抗原决定簇的鼠单抗(PAb6)发生反应，该蛋白映射了病毒基因组上0.42个MAP单位的5‘。P72蛋白是一种丰富的碱性(PI>；7)细胞质蛋白，存在于SV40转化和未转化的亲本细胞以及来自正常人类和肿瘤组织的细胞系中。通过二维凝胶分析和Western印迹分析，PAb6鉴定的P72蛋白与细胞质信使RNA分子Poly(A)+链上的72000molw蛋白PABP没有区别。在正常人外周血单个核细胞中，在T细胞特异性有丝分裂凝集素植物血凝素的刺激下，P72的合成和胞浆积聚发生在G0、→、G1期转变的很早。P72蛋白在增殖和分化的人早幼粒细胞HL60细胞中也有表达，表明该蛋白的表达并不严格限于周期细胞。
In this communication, we have studied a 72,000 mol w (p72) host protein which reacts with a mouse monoclonal antibody (PAb6) directed against antigenic determinants on the Simian virus 40 (SV40) large T antigen protein that map 5′ of 0.42 map units on the viral genome. The p72 protein is an abundant basic (pI>7) cytoplasmic protein found in both SV40-transformed and untransformed parental cells and in cell lines derived from normal human and tumor tissue. By two-dimensional gel analysis and Western blot analysis the p72 protein identified by PAb6 is indistinguishable from the 72,000 mol w protein PABP associated with the poly(A)+tract of cytoplasmic messenger RNA molecules. In normal human peripheral blood mononuclear cells stimulated to proliferate with the T-cell-specific mitogenic lectin phytohemagglutinin the synthesis and cytoplasmic accumulation of p72 occurs very early during the G0→G1-phase transition. The p72 protein is also expressed in proliferating and differentiated human promyelocytic HL60 cells indicating that the expression of this protein is not strictly limited to cycling cells.