SEQUENCE INVESTIGATION OF THE MAJOR GASTROINTESTINAL TUMOR-ASSOCIATED ANTIGEN GENE FAMILY, GA733
SEQUENCE INVESTIGATION OF THE MAJOR GASTROINTESTINAL TUMOR-ASSOCIATED ANTIGEN GENE FAMILY, GA733
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DOI：
10.1073/pnas.86.1.27
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发表时间：
1989-01-01
影响因子：
11.1
通讯作者：
KOPROWSKI, H
中科院分区：
文献类型：
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作者：
LINNENBACH, AJ;WOJCIEROWSKI, J;KOPROWSKI, H
从SW948人结直肠癌细胞系中纯化出单克隆抗体定义的肿瘤相关抗原GA733，并测定了其部分氨基酸序列。通过使用合成寡核苷酸探针，从总人类基因组文库中分离出两种重组体。我们证明了 GA733 基因家族的存在。其中一个基因组分离株被证明是无内含子基因，其在胰腺癌细胞系和胎盘中转录。观察到 GA733 蛋白含有与甲状腺球蛋白中出现 10 次和 HLA-DR 相关不变链中出现一次的重复单元同源的序列。发现了与.alpha在进化上更为遥远的关系。白细胞介素2生长因子受体链。
The monoclonal antibody-defined, tumor associated antigen GA733 was purified from the SW948 human colorectal carcinoma cell line and its partial amino acid sequence was determined. By using a synthetic oligonucleotide probe, two recombinants were isolated from a total human genomic library. We prove the existence of a family of GA733 genes. One of the genomic isolates id demonstrated to be an intronless gene, which is transcribed in pancreatic carcinoma cell lines and in placenta. The GA733 proteins were observed to contain sequences homologous to a repeat unit occurring 10 times in thyroglobulin and once in the HLA-DR-associated invariant chain. A more evolutionarily distant relationship was found with the .alpha. chain of the interleukin 2 growth factor receptor.