17β-Estradiol Transport and Metabolism in Human Red Blood Cells
17β-Estradiol Transport and Metabolism in Human Red Blood Cells
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人红细胞中 17β-雌二醇的转运和代谢
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
M. K. Jacobsohn
中科院分区：
文献类型：
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作者：
G. Jacobsohn;E. Siegel;M. K. Jacobsohn
尝试测量红细胞作为类固醇代谢组织的功能表明，细胞膜起着重要作用。在与红细胞相关的17²-雌二醇中，2/3与细胞膜结合，1/3在可溶性部分。这种结合似乎是非特异性的，与孵育量线性相关。2℃时的结合比26℃时更广泛。用等渗氯化钠溶液连续洗涤红细胞增加了它们结合17²-雌二醇的能力。在等渗盐培养基中培养的红细胞去除了85.1±3.2% （sd）的总雌激素，在37℃下短至15秒，长至19小时的孵育时间内，这个值保持在很窄的范围内。在同源血浆中孵育，培养基中只有8-12%的雌激素与细胞相关。盐溶液和血浆细胞结合的8倍差异可归因于与血浆蛋白的竞争。血浆蛋白的存在减少但不阻止17²-…的活性。
Attempts to measure the function of red blood cells as steroid metabolizing tissue showed that the membrane plays an important role. Of the 17²-estradiol associated with red cells, 2/3 was bound to the membrane while 1/3 was in the soluble fraction. The binding appears to be nonspecific and linearly related to the amount incubated. Binding was more extensive at 2 C than at 26 C. Successive washes of red cells with isotonic sodium chloride solution increased their ability to bind 17²-estradiol. Red cells incubated in isotonic salt media removed 85.1 ± 3.2% (sd) of the total estrogens present and this value remained within narrow limits for incubation times as short as 15 s and as long as 19 h at 37 C. When incubated in homologous plasma, only 8–12% of estrogens in the media became associated with cells. The eight-fold difference in cell binding between salt solution and plasma can be attributed to competition with plasma proteins. Presence of plasma proteins diminishes but does not prevent activity of 17²-...