The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma
The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma
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microRNA加工酶：Drosha和Dicer可以预测鼻咽癌的预后
DOI：
10.1007/s00432-011-1058-1
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发表时间：
2012-01-01
影响因子：
3.6
通讯作者：
Li, Guiyuan
中科院分区：
文献类型：
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作者：
Guo, Xiaofang;Liao, Qianjin;Li, Guiyuan
目的在多种人类癌症中已经观察到microRNA（miRNA）代谢的失调，但是负责产生miRNA的酶的表达模式在很大程度上仍然未被探索。在这项研究中，我们调查的两个最重要的酶的miRNA机制，Drosha和Dicer，这是密切相关的鼻咽癌（NPC）和患者survival.MethodsDicer和Drosha mRNA水平的表达谱采用实时定量逆转录聚合酶链反应（qRT-PCR）检测24例NPC组织，7例正常鼻咽上皮（NPE）和NPC细胞系。此外，使用NPC组织芯片（TMA）通过免疫组织化学（IHC）检测蛋白水平，其中包括251例NPC和105例NPE病例。对于一些无法联系的NPC患者，仅146例患者的生存数据可用。结果Dicer和Drosha mRNA在鼻咽癌组织和细胞系中的平均表达水平均显著低于对照组。Dicer和Drosha蛋白在鼻咽癌组织中的低表达与患者的无进展生存期（PFS）和总生存期（OS）显著相关。结论Drosha和Dicer蛋白在鼻咽癌组织中的表达与患者的PFS和OS显著相关。因此，我们假设Dicer和Drosha的表达水平可以用作NPC的潜在预后生物标志物。
PurposeDysregulation of microRNA (miRNA) metabolism has been observed in a variety of human cancers, but the expression patterns of the enzymes responsible for generating miRNAs remain largely unexplored. In this study, we investigated the expression profiles of the two most important enzymes of the miRNA machinery, Drosha and Dicer, which were closely correlated with nasopharyngeal carcinoma (NPC) and patient survival.MethodsDicer and Drosha mRNA levels were detected by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) using 24 NPC tissues, 7 normal nasopharyngeal epithelium samples (NPE) and NPC cell lines. In addition, protein levels were detected by immunohistochemistry (IHC) using an NPC tissue microarray (TMA), which include 251 NPC and 105 NPE cases. For some NPC patients can not be contacted, the survival data were available only for 146 patients. Kaplan–Meier analysis was performed, and the chi-square and log-rank tests were used to detect significance levels using SPSS 15.0 software.ResultsThe mean level of Dicer and Drosha mRNA were significantly down-regulated in NPC tissue specimens and cell lines when compared with controls. The low levels of Dicer and Drosha protein were frequently seen in NPC, and the low expression of Dicer and Drosha protein was significantly correlated with shorter progression-free survival (PFS) and overall survival (OS) of NPC patients.ConclusionsWe observed that Drosha and Dicer expression was dysregulation in NPC compared with healthy control samples and was significantly correlated with shorter PFS and OS of NPC patients. Therefore, we hypothesise that the expression levels of Dicer and Drosha could be used as potential prognostic biomarkers for NPC.