Enhancing the Enzymatic Hydrolysis of Corn Stover by an Integrated Wet-milling and Alkali Pretreatment
Enhancing the Enzymatic Hydrolysis of Corn Stover by an Integrated Wet-milling and Alkali Pretreatment
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综合湿磨和碱预处理增强玉米秸秆的酶解
DOI：
10.1007/s12010-009-8736-3
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发表时间：
2010-04-01
影响因子：
3
通讯作者：
Ouyang, Pingkai
中科院分区：
文献类型：
--
作者：
He, Xun;Miao, Yelian;Ouyang, Pingkai
对玉米秸秆进行湿法粉碎和碱预处理后再进行酶解处理。研究了氢氧化钠浓度对玉米秸秆的晶体结构、化学组成和还原糖得率的影响，并对其提高还原糖得率的机理进行了探讨。实验结果表明，1%NaOH溶液处理玉米秸秆1h，玉米秸秆的晶体结构被破坏，木质素被去除，而纤维素和半纤维素保留不变，当NaOH浓度从0%增加到1%时，玉米秸秆还原糖的得率从20.2%提高到46.7%。经1%NaOH处理的玉米秸秆的综纤维素转化率为55.1%。还原糖产量的增加与玉米秸秆的结晶结构破坏和脱木素有关。结果表明，预处理显著提高了玉米秸秆中纤维素和半纤维素向糖的转化。
An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yield by the pretreatment was discussed. The experimental results showed that the crystalline structure of corn stover was disrupted, and lignin was removed, while cellulose and hemicellulose were retained in corn stover by the pretreatment with 1% NaOH in 1 h. The reducing-sugar yield from the pretreated corn stovers increased from 20.2% to 46.7% when the NaOH concentration increased from 0% to 1%. The 1% NaOH pretreated corn stover had a holocellulose conversion of 55.1%. The increase in reducing-sugar yield was related to the crystalline structure disruption and delignification of corn stover. It was clarified that the pretreatment significantly enhanced the conversion of cellulose and hemicellulose in the corn stover to sugars.