Inhibitors of nitric oxide synthetase prevent castor‐oil‐induced diarrhoea in the rat
Inhibitors of nitric oxide synthetase prevent castor‐oil‐induced diarrhoea in the rat
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一氧化氮合成酶抑制剂可预防大鼠蓖麻油引起的腹泻
DOI：
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发表时间：
1993
影响因子：
7.3
通讯作者：
F. Capasso
中科院分区：
文献类型：
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作者：
N. Mascolo;A. Izzo;F. Barbato;F. Capasso
1 .蓖麻油（口服2ml）给药后3小时使大鼠出现大量腹泻。2用NO合成抑制剂NG -硝基- 1 -精氨酸甲酯（1 - NAME, 1 - 25 mg kg - 1）和NG -单甲基- 1 -精氨酸（1 - NMMA, 2.5-100 mg kg - 1）对大鼠进行预处理（腹腔，i.p），可以抑制或预防蓖麻油诱导的腹泻。l -精氨酸（150-600 mg kg - 1, i.p）给以l - NAME 10 mg kg - 1预处理的大鼠，以剂量相关的方式显著降低了l - NAME的抗腹泻活性。d‐精氨酸（900 mg kg - 1）不改变l‐NAME的保护作用。1 - NAME预处理（2.5 ~ 25 mg kg - 1）可降低蓖麻油诱导的肠液积聚和Na+分泌。l‐精氨酸（600 mg kg - 1）而非d‐精氨酸（900 mg kg - 1）抵消了l‐NAME （10 mg kg - 1）的抑制作用。4 l‐NAME（10和25 mg kg−1）对正常大鼠或给予蓖麻油的大鼠的肠道运输没有显著影响。这些结果证明，一氧化氮（NO）在蓖麻油引起的腹泻中起重要作用。
1 Castor oil (2 ml orally) produced copious diarrhoea in rats 3 h after its administration. 2 Pretreatment (intraperitoneal, i.p.) of rats with the NO synthesis inhibitors NG‐nitro‐l‐arginine methyl ester (l‐NAME, 1–25 mg kg−1) and NG‐monomethyl‐l‐arginine (l‐NMMA, 2.5–100 mg kg−1) inhibited or prevented castor‐oil‐induced diarrhoea. l‐Arginine (150–600 mg kg−1, i.p.) administered to rats pretreated with l‐NAME 10 mg kg−1, drastically reduced the antidiarrhoeal activity of l‐NAME in a dose‐related manner. d‐Arginine (900 mg kg−1) did not modify the protection by l‐NAME. 3 Pretreatment (i.p.) of rats with l‐NAME (2.5–25 mg kg−1) decreased the intestinal fluid accumulation and Na+ secretion induced by castor oil. l‐Arginine (600 mg kg−1) but not d‐arginine (900 mg kg−1) counteracted the inhibitory effect of l‐NAME (10 mg kg−1). 4 l‐NAME (10 and 25 mg kg−1) had no significant effect on the intestinal transit in normal rats or those given castor oil. 5 These results provide evidence that nitric oxide (NO) could play an important role in castor‐oil‐induced diarrhoea.
DOI：
10.1073/pnas.85.22.8664
发表时间：
1988-11-01
影响因子：
11.1
作者：
SAKUMA, I;STUEHR, DJ;LEVI, R
通讯作者：
LEVI, R