Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells.
Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells.
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DOI：
10.1186/1471-213x-1-11
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发表时间：
2001
影响因子：
--
通讯作者：
Elder RF
中科院分区：
文献类型：
--
作者：
Bukovsky A;Caudle MR;Keenan JA;Upadhyaya NB;Van Meter SE;Wimalasena J;Elder RF
间充质-上皮相互作用在上皮组织的生理学和病理学中发挥重要作用。间充质细胞要么与上皮基底膜 [周细胞和血管周围单核细胞衍生细胞 (MDC)] 结合，要么驻留在上皮内（MDC 和 T 细胞）。尽管上皮内间充质细胞被认为有助于上皮生理学，但它们与上皮细胞分化的特定步骤的关系、它们之间的相互作用以及它们的命运仍不清楚。我们研究了子宫外宫颈复层上皮中的间充质细胞及其产物（免疫球蛋白）的表位，这是从干细胞完全分化为衰老细胞的原型之一。血管周围 CD14 原始 MDC 与基底（干）上皮细胞相关。微脉管系统的 Thy-1 周细胞分泌细胞间囊泡，与表达 I 类 MHC 的 Ki67 有丝分裂后上皮细胞相关。上皮内 T 细胞与副基底细胞中的遮盖型 MDC [树突状细胞 (DC) 前体] 相关，并在进入中间（成熟）上皮层后表现出碎片化。成熟的DC分泌CD68并在到达中间层后表现出碎片化。在每一层的顶部检测到 IgM 的结合：在上副基底细胞、上中间细胞和大多数表面上皮细胞中。 IgG 局限于整个表层。这些数据表明，间充质细胞（MDC、周细胞、T细胞）和免疫球蛋白（IgM、IgG）的系统发育和个体发育层次结构伴随着上皮细胞从未成熟向成熟和衰老表型的分化。对间充质细胞参与组织稳态调节的进一步研究可能会带来预防和治疗以永久性组织不成熟（肌营养不良）或加速衰老（退行性疾病）为特征的组织功能障碍的新方法。
Mesenchymal-epithelial interactions play an important role in the physiology and pathology of epithelial tissues. Mesenchymal cells either associate with epithelium basement membrane [pericytes and perivascular monocyte-derived cells (MDC)] or reside within epithelium (MDC and T cells). Although intraepithelial mesenchymal cells were suggested to contribute to the epithelium physiology, their association with particular steps in differentiation of epithelial cells, interactions among themselves, and their fate remain unclear. We studied epitopes of mesenchymal cells and their products (immunoglobulins) in stratified epithelium of uterine ectocervix, which is one of the prototypes of complete cellular differentiation from stem into the aged cells. Perivascular CD14 primitive MDC associated with basal (stem) epithelial cells. Thy-1 pericytes of microvasculature secreted intercellular vesicles, which associated with Ki67 postmitotic epithelial cells expressing MHC class I. Intraepithelial T cells showed an association with veiled type MDC [dendritic cell (DC) precursors] among parabasal cells, and exhibited fragmentation after entering intermediate (mature) epithelial layers. Mature DC secreted CD68 and exhibited fragmentation after reaching mid intermediate layers. Binding of IgM was detected at the top of each layer: in the upper parabasal, upper intermediate, and most surface epithelial cells. IgG was confined to the entire superficial layer. These data suggest that the phylogenetically and ontogenetically developed hierarchy of mesenchymal cells (MDC, pericytes, T cells) and immunoglobulins (IgM, IgG) accompanies differentiation of epithelial cells from immature into the mature and aged phenotype. Further studies of an involvement of mesenchymal cells in the regulation of tissue homeostasis may bring novel approaches to the prevention and therapy of tissue dysfunctions characterized by permanent tissue immaturity (muscular dystrophy) or accelerated aging (degenerative diseases).