Interferon-gamma regulation of human renal cortical epithelial cell-derived monocyte chemotactic peptide-1.
Interferon-gamma regulation of human renal cortical epithelial cell-derived monocyte chemotactic peptide-1.
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干扰素-γ对人肾皮质上皮细胞来源的单核细胞趋化肽-1的调节。
DOI：
10.1038/ki.1993.211
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发表时间：
1993
影响因子：
19.6
通讯作者：
Kunkel,SL
中科院分区：
文献类型：
--
作者：
Schmouder,RL;Strieter,RM;Kunkel,SL
干扰素γ对人肾皮质上皮细胞来源的单核细胞趋化肽-1的调控。在急性炎症期间导致单核细胞/巨噬细胞向小管间质转移的趋化信号尚不清楚。单核细胞趋化肽-1 （MCP-1）是最近被发现的一种趋化细胞因子，在肾脏炎症中可能既是一种有效的单核细胞趋化素，也是一种激活剂。我们研究了培养的肾小管源性皮质上皮细胞（RCEC）可能被刺激产生MCP-1的促炎条件。用IL-1β (0.01 ~ 1.0 ng/ml)、TNF （0.1 ~ 10 ng/ml）、LPS（0.1 ~ 10µg/ml）或INF-γ (10 ~ 1000 U/ml)以剂量-时间依赖性方式刺激RCEC。用IL-1β3或INF-γ刺激RCEC的条件培养基产生单核细胞趋化活性，该活性被MCP-1中和抗体显著抑制。用IL-1β或INF-γ刺激RCEC导致MCP-1 mRNA稳态水平显著（4- 5倍）增加。在IL-1β或INF-γ刺激后，RCEC条件培养基中的MCP-1抗原肽显著高于对照组（2- 2.5倍）。相反，在RCEC中，INF-γ不刺激白细胞介素-8 （IL-8）的产生，IL-8是一种中性粒细胞趋化细胞因子。因此，肾小管细胞的趋化因子信号库可能受到INF-γ的选择性控制。
Interferon-γ regulation of human renal cortical epithelial cell-derived monocyte chemotactic peptide-1. The chemoattractant signal(s) that results in the transmigration of monocytes/macrophage into the tubulointerstitium during acute inflammation is not known. Monocyte chemotactic peptide-1 (MCP-1), a recently described chemotactic cytokine, may function as both a potent monocyte chemotaxin and activator in renal inflammation. We have studied the proinflammatory conditions in which cultured human renal cortical epithelial cells (RCEC) of tubular origin may be stimulated to produce MCP-1. RCEC were stimulated in a dose-time dependent manner with: IL-1β (0.01 to 1.0 ng/ml), TNF (0.1 to 10 ng/ml), LPS (0.1 to 10 µg/ml) or INF-γ (10–1000 U/ml). Conditioned media from RCEC stimulated with either IL-1β3 or INF-γ produced a monocyte chemoattractant activity which was significantly suppressed with neutralizing antibody to MCP-1. Stimulation of RCEC with either IL-1β or INF-γ resulted in a significant (4- to 5-fold) increase in steady state levels of MCP-1 mRNA. MCP-1 antigenic peptide in RCEC conditioned media was significantly increased over control (2- to 2.5-fold) after stimulation with either IL-1β or INF-γ. In contrast, production of interleukin-8 (IL-8), a neutrophil chemotactic cytokine, was not stimulated by INF-γ in RCEC. Thus, the chemokine signaling repertoire of renal tubule cells may be selectively controlled by INF-γ.