Suppression of plant defenses by a Myzus persicae (green peach aphid) salivary effector protein.
Suppression of plant defenses by a Myzus persicae (green peach aphid) salivary effector protein.
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DOI：
10.1094/mpmi-01-14-0018-r
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发表时间：
2014-07
期刊：
影响因子：
--
通讯作者：
Jander G
中科院分区：
文献类型：
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作者：
Elzinga DA;De Vos M;Jander G
蚜虫和它们的寄主植物之间的复杂相互作用是物种特有的，涉及多层识别和防御。在韧皮部取食过程中释放到植物体内的蚜虫唾液蛋白可能是这些相互作用的中介。在一种鉴定促进寄主植物取食的蚜虫效应物的方法中，在烟草中瞬时表达了11种桃蚜(Myzus Persicae)唾液蛋白和棉铃虫(Buchnera Aphidicola)的GroEL蛋白。虽然两种唾液蛋白促进了烟粉虱的繁殖，但另外三种蛋白和GroEL的表达显著抑制了烟粉虱上的蚜虫繁殖。这些效应在稳定的转基因拟南芥(Arabiopsis Thaliana)植株中被概括。进一步用拟南星天牛表达Mp55的实验结果表明，4-甲氧基吲哚-3-甲基硫代葡萄糖苷、骨痂和过氧化氢的积累较低。Mp55是一种唾液蛋白，可促进蚜虫繁殖。在选择性试验中，表达Mp55的植株也更吸引蚜虫。利用RNA干扰技术抑制烟粉虱中Mp55基因的表达减少了烟粉虱、烟粉虱和烟叶上的蚜虫繁殖量。综上所述，这些结果证明了Mp55，一种分子功能尚不清楚的蛋白质，在烟草根结线虫与其寄主植物的相互作用中发挥了作用。
The complex interactions between aphids and their host plant are species-specific and involve multiple layers of recognition and defense. Aphid salivary proteins, which are released into the plant during phloem feeding, are a likely mediator of these interactions. In an approach to identify aphid effectors that facilitate feeding from host plants, eleven Myzus persicae (green peach aphid) salivary proteins and the GroEL protein of Buchnera aphidicola, a bacterial endosymbiont of this aphid species, were expressed transiently in Nicotiana tabacum (tobacco). Whereas two salivary proteins increased aphid reproduction, expression of three other aphid proteins and GroEL significantly decreased aphid reproduction on N. tabacum. These effects were recapitulated in stable transgenic Arabidopsis thaliana (Arabidopsis) plants. Further experiments with A. thaliana expressing Mp55, a salivary protein that increased aphid reproduction, showed lower accumulation of 4-methoxyindol-3-ylmethylglucosinolate, callose, and hydrogen peroxide in response to aphid feeding. Mp55-expressing plants also were more attractive for aphids in choice assays. Silencing Mp55 gene expression in M. persicae using RNA interference approaches reduced aphid reproduction on N. tabacum, A. thaliana, and Nicotiana benthamiana. Together, these results demonstrate a role for Mp55, a protein with as yet unknown molecular function, in the interaction of M. persicae with its host plants.