A mitochondrion as the structural basis of the formation of a cell-type-specific organelle in Dictyostelium development
A mitochondrion as the structural basis of the formation of a cell-type-specific organelle in Dictyostelium development
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DOI：
10.1007/bf01287413
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发表时间：
1998-01-01
期刊：
影响因子：
2.9
通讯作者：
Maeda, Y
中科院分区：
文献类型：
--
作者：
Matsuyama, SI;Maeda, Y
线粒体作为一种自我繁殖和呼吸的细胞器，一直受到人们的关注。我们在这里报道，线粒体可能参与形成细胞类型特有的细胞器，与高尔基复合体结合。盘基网柄菌在发育过程中，前柄细胞和后前孢子细胞两类细胞形成极化细胞团。前孢子分化的特征是细胞质中存在独特的空泡，称为前孢子特有的空泡。因此，PSV是了解该生物体细胞分化的结构基础的最基本的细胞器。在分化前孢子细胞的过程中，线粒体进行了显著的转化，形成了一种液泡(M液泡)。用PSV特异性抗体免疫细胞化学方法证明PSV抗原(C-IO)定位于PSV的M空泡和衬膜中。有趣的是，在与M-液泡融合的高尔基体池中也发现了C-10抗原。基于这些发现，我们在这里提出了一个有希望的模型，该模型表明线粒体和高尔基体池可能共同参与PSV的形成。这个模型将为线粒体在细胞分化中的功能提供一个新的方面。
The mitochondrion has been mainly given attention as a self-reproductive and respiratory organelle. We report here that the mitochondrion may participate in the formation of a cell-type-specific organelle, coupling with the Golgi complex. During the development of Dictyostelium discoideum, the two types of cells, i.e., the anterior prestalk cells and the posterior prespore cells form a polarized cell mass. Prespore differentiation is characterized by the presence of unique vacuoles named PSVs (prespore-specific vacuoles) in the cytoplasm. Thus the PSV is the most essential organelle to understand the structural basis of cell differention in this organism. In differentiating prespore cells, the mitochondrion exerts a remarkable transformation to form a sort of vacuole (M-vacuole). Using a PSV specific antibody, it was immunocytochemically shown that a PSV antigen (C-IO) is localized in the M-vacuole as well as in the lining membrane of PSV. Interestingly, the C-10 antigen was also noticed in the Golgi cisternae that had fused with M-vacuole. Based on these findings, we propose here a promising model which suggests how both mitochondria and Golgi cisternae may be coordinately involved in the PSV formation. This model will provide a new aspect of mitochondrial functions in cell differentiation.