Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules.
Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules.
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DOI：
10.1039/c4lc00895b
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发表时间：
2014-12-07
期刊：
影响因子：
6.1
通讯作者：
Song YA
中科院分区：
文献类型：
--
作者：
Syed A;Mangano L;Mao P;Han J;Song YA
在这项工作中，我们描述了一种新颖而简单的胶体硅珠自组装，以在两个微通道之间创建纳米流体结。纳米孔膜用于诱导微通道内的离子浓度极化，这种电动预浓缩系统可以在5分钟内将DNA样品快速浓缩~ 1700倍，将蛋白质样品快速浓缩~ 100倍。
In this work we describe a novel and simple self-assembly of colloidal silica beads to create nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ∼1700 times and protein samples by ∼100 times within 5 minutes.