Plasmid-determined enzymatic degradation of nylon oligomers
Plasmid-determined enzymatic degradation of nylon oligomers
复制标题
尼龙低聚物的质粒决定的酶降解
DOI：
10.1128/jb.155.1.22-31.1983
复制
发表时间：
1983
影响因子：
3.2
通讯作者：
H. Okada
中科院分区：
文献类型：
--
作者：
S. Negoro;T. Taniguchi;M. Kanaoka;H. Kimura;H. Okada
将黄杆菌KI72质粒pOAD2上的尼龙低聚物（6-氨基己酸环二聚体）降解基因克隆到大肠杆菌载体pBR322中。其中6-氨基己酸线性低聚物水解酶结构基因通过构建不同的缺失质粒，将大肠杆菌的lacUV5启动子片段插入缺失质粒，确定了缺失基因的位点。通过DNA-DNA杂交实验，在pOAD2上检测到两种重复序列（RS-I和RS-II）。这些重复序列在pOAD2上出现了5次（RS-I）或2次（RS-II）。其中一个RS-II区域与水解酶结构基因重叠。
The nylon oligomer (6-aminohexanoic acid cyclic dimer) degradation genes on plasmid pOAD2 of Flavobacterium sp. KI72 were cloned into Escherichia coli vector pBR322. The locus of one of the genes, the structural gene of 6-aminohexanoic acid linear oligomer hydrolase, was determined by constructing various deletion plasmids and inserting the lacUV5 promoter fragment of E. coli into the deletion plasmid. Two kinds of repeated sequences (RS-I and RS-II) were detected on pOAD2 by DNA-DNA hybridization experiments. These repeated sequences appeared five times (RS-I) or twice (RS-II) on pOAD2. One of the RS-II regions and the structural gene of the hydrolase overlapped.