VANADYL AND VANADATE INHIBIT CA-2+ TRANSPORT-SYSTEMS OF THE ADIPOCYTE PLASMA-MEMBRANE AND ENDOPLASMIC-RETICULUM
VANADYL AND VANADATE INHIBIT CA-2+ TRANSPORT-SYSTEMS OF THE ADIPOCYTE PLASMA-MEMBRANE AND ENDOPLASMIC-RETICULUM
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DOI：
10.1016/0003-9861(85)90593-4
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发表时间：
1985-01-01
影响因子：
3.9
通讯作者：
MCDONALD, JM
中科院分区：
文献类型：
--
作者：
DELFERT, DM;MCDONALD, JM
钒酸盐和钒酸盐对细胞代谢有许多胰岛素模拟作用，也被证明可以改变细胞Ca2+通量。在本报告中，钒酸盐和钒酰，像胰岛素一样，被证明可以抑制大鼠脂肪细胞的质膜(Ca2+ + Mg2+)- atp酶/Ca2+运输系统以及内质网Ca2+运输。在浓度为30和33 μ m时，钒酸盐和钒酰对内质网Ca2+运输的抑制作用达到一半（50%）。M,分别。钒酸盐和钒酰对质膜Ca2+运输的抑制作用较弱，I50值分别为144和92 .mu。M,分别。在钙调素刺激和非钙调素刺激的Ca2+运输条件下，质膜Ca2+运输的I50值是相似的。两种离子对Ca2+输运动力学参数的主要影响是两种输运系统的Vmax均显著降低43-46%。在这些钒离子抑制质膜和内质网(Ca2+ + Mg2+)- atp酶/Ca2+泵后，细胞内Ca2+的增加可能导致，至少部分地，在细胞代谢中观察到胰岛素模拟的改变。
Vanadate and vanadyl have many insulin-mimetic effects on cellular metabolism and also have been shown to alter cellular Ca2+ fluxes. In this report, vanadate and vanadyl, like insulin, are shown to inhibit the plasma membrane (Ca2+ + Mg2+)-ATPase/Ca2+ transport system as well as Ca2+ transport by endoplasmic reticulum from rat adipocytes. Ca2+ transport by the endoplasmic reticulum was inhibited half-maximally (I50) by vanadate and vanadyl at concentrations of 30 and 33 .mu.M, respectively. Inhibition of the plasma membrane Ca2+ transport by vanadate and vanadyl was less sensitive, with I50 values of 144 and 92 .mu.M, respectively. These I50 values for plasma membrane Ca2+ transport were similar when measured under conditions of calmodulin-stimulated and noncalmodulin-stimulated Ca2+ transport. The predominant effect of both ions on the kinetic parameters of Ca2+ transport was a substantial decrease in the Vmax by 43-46% for both transport systems. An increase in intracellular Ca2+ following the inhibition of the (Ca2+ + Mg2+)-ATPase/Ca2+ pump in the plasma membrane and endoplasmic reticulum by these vanadium ions may result, at least in part, in the observed insulin-mimetic alterations in cellular metabolism.