Storage of platelet concentrates after high-dose ultraviolet B irradiation.
Storage of platelet concentrates after high-dose ultraviolet B irradiation.
复制标题
高剂量紫外线 B 照射后血小板浓缩液的储存。
DOI：
10.1046/j.1537-2995.1991.31691306243.x
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Buchholz,DH
中科院分区：
文献类型：
--
作者：
Snyder,EL;Beardsley,DS;Smith,BR;Horne,W;Johnson,R;Wooten,T;Napychank,PA;Male,P;Buchholz,DH
中波紫外线(UVB)照射浓缩血小板(PC)可预防输血后同种异体免疫反应的发生。由于在血液中心或医院进行的辐射可能与输血前辐射后的可变延迟有关，因此在UVB辐射后存储PC的能力变得重要。我们已经研究了每平方厘米10000MJ的UVB剂量，这是我们机构用于UVB临床试验的剂量，在照射后汇集和储存长达96小时的PC上进行了效果研究。结果表明，保存96h后，虽然pH、血小板计数、白细胞计数、乳酸脱氢酶释放百分率、β-血栓球蛋白等指标无明显变化，但形态评分和渗透复原率明显下降。然而，在储存24小时后，这些变化并不明显。同样，免疫反应膜糖蛋白(GP)Ib在储存96小时后下降了60%，但在储存48小时后看不到这些变化。在96小时的保存过程中，两组的GPIIb/IIIa水平均无明显变化。在计算机分析的双向聚丙烯酰胺凝胶电泳仪上，经10000MJ/cm2辐照并储存72小时的PC与未辐照的年龄匹配的对照PC相比，有超过50个血小板蛋白发生了变化。结果表明，在10,000 mJ/cm2的UVB照射下，PC在短期(24-48小时)储存后不会导致明显的血小板变性，但在长期(72-96小时)储存后可能是有害的。此外，中波紫外线诱导了血小板中轮蛋白特异性的改变，如改变GPlb，同时保持GPllb/111a相对完整。
Ultraviolet B (UVB) irradiation of platelet concentrates (PCs) may prevent the development of posttransfusion HLA alloimmunization. As irradiation performed in a blood center or a hospital will probably be associated with a variable postirradiation delay before transfusion, the ability to store PCs after UVB irradiation becomes important. The effects have been studied of a UVB dose of 10,000 mJ per cm2, the dose used in our institution for UVB clinical trials, on PCs pooled and stored for up to 96 hours after irradiation. Results showed that after 96 hours of storage, though there were no changes in pH, platelet count, white cell count, percent discharge of lactate dehydrogenase, or beta‐thromboglobulin, there were significant decreases in morphology score and osmotic recovery. These changes, however, were not evident after 24 hours of storage. Similarly, there was a 60‐percent decrease in immunoreactive membrane glycoprotein (GP) Ib after 96 hours of storage, but these changes were not seen after 48 hours of storage. No changes were seen in levels of GPIIb/IIIa in either group during the 96 hours of storage. On computer‐analyzed two‐dimensional polyacrylamide gel electrophoresis, PCs irradiated at 10,000 mJ per cm2 and stored for 72 hours had changes in over 50 platelet proteins as compared to those proteins in nonirradiated age‐matched control PCs. It can be concluded that UVB irradiation of PCs at 10,000 mJ per cm2 does not lead to significant platelet deterioration after short‐term storage (24‐48 hours) but is likely to be deleterious after long‐term (72‐96 hours) storage. Moreover, UVB induces rotein‐specific alterations stora in the platelet, such as altering GPlb, while leaving GPllb/llla relatively intact.